FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780
Total Bases258 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT53129.831460674157302No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT43624.49438202247191No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC1538.595505617977528No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT975.44943820224719No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC271.5168539325842696No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCTCACAGAGAC211.1797752808988764No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACAAAGTCTCT140.7865168539325843No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG130.7303370786516854No Hit
GCAAAAGTTCTCAGGGATGTCCGATCGAGCAACTCTGCGTTGATACCACC120.6741573033707865No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAACATGGGCA120.6741573033707865No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG110.6179775280898876No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACCCCATG110.6179775280898876No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG100.5617977528089888No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCTCACAGAGAC100.5617977528089888No Hit
GCAAAAGTTCTCAGGGATGTCCGATCGAGCAACTCTGCGTTGATACCACT80.44943820224719105No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC70.39325842696629215No Hit
CAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG60.33707865168539325No Hit
GAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG50.2808988764044944No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG50.2808988764044944No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT40.22471910112359553Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTCCCCATGTACTCTGCGT40.22471910112359553No Hit
GCAAAAGTTCTCAGAGATGTGTAAACGCAGCTCACAGAGACCAAGTCTCT40.22471910112359553No Hit
GCAAAAGTTCTAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.22471910112359553No Hit
CGCCAGGCAGGAATCACACTGACGACATGGTTCTACAACATGGGCAAAAG40.22471910112359553No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCCCATGTACTCTG40.22471910112359553No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACAAAGTCTCT40.22471910112359553No Hit
GCAAAAGTTCTCAGGGATGTCCCGATCGAGCAACTCTGCGTTGATACCAC40.22471910112359553No Hit
CAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.22471910112359553No Hit
GAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGACC30.16853932584269662No Hit
AACTCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTCT30.16853932584269662No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACTGCTTC30.16853932584269662Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAATTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.16853932584269662No Hit
GCAAAAGTTCTCAGAGATGTGTAGCGCAGCTCACAGAGACCAAGTCTCTG30.16853932584269662No Hit
AACTCCTGTTTAGGCCACTGATAGGATCACAGAGACCAAGTCTCTGCTAC30.16853932584269662No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG30.16853932584269662No Hit
GCAAAAGTTCTAAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT30.16853932584269662No Hit
GCAAAAGTTCTCAGGGATGTCGAGCAACTCTGCGTTGATACCACCCCATG30.16853932584269662No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAACTCTCT30.16853932584269662No Hit
GCAAAAGTTTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.16853932584269662No Hit
GCAAAAGTTCTCAGGGATGTCCGATCGAGCAACTCCCCATGTACTCTGCG30.16853932584269662No Hit
GCAAAAGTTCTAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.16853932584269662No Hit
AACTCCTGTTTAGGCCTCTCTCCTCATTCTAAACAACTCACAGAGACCAA30.16853932584269662No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCGGCTCACAGAGACCAAGTCTCT30.16853932584269662No Hit
AGCACTTGAATCAACAATGATCAGCATCACAGAGACCAAGTCTCTGCTAC20.11235955056179776No Hit
GCAAAAGTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG20.11235955056179776No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTATAGGATCACAGAGACCAAGTCT20.11235955056179776No Hit
GCAAAATTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAACGCAGCTCACAGAGAC20.11235955056179776No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCACT20.11235955056179776No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGTT20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCCGATCGAGCAACTCTGCGTTGATACCATC20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATATGTAAGCGCAGCTCACAGAGACCAAGTCTCT20.11235955056179776No Hit
AACTCCTGTTTAGGCCTGCGGCCACTGATAGGATCACAGAGACCAAGTCT20.11235955056179776No Hit
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG20.11235955056179776No Hit
GCAAAAGTTCTAGGGATGTCCGAGATGTGTAAGCGCAGCTCACAGAGACC20.11235955056179776No Hit
AACCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCACTCACAGAGACCAAGTCTCTG20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTAGAAGAG20.11235955056179776Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCGATCGAGCAACTCTGCGTTGATACCCCA20.11235955056179776No Hit
GCAAAAGTTCTCAGGAATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGTTCACAGAGACCAAGTCTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTGAGATGTGTAAGCGCAGCTCACAGAGACCA20.11235955056179776No Hit
AACTCCTGTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCA20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCCGATCAGCAACTCTGCGTTGATACCACCC20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTGTAAACGCAGCTCACAGAGACCAAGTCTCT20.11235955056179776No Hit
GCAAAAGTTCTCGGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGGAATGTGTAAGCGCACTCACAGAGACCAAGTCTCTG20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGGCGTGTTGATGAGGGGATGGCGTGTTGATGA20.11235955056179776No Hit
ACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCCCACTGACGACATGGTTCTACAACATGGG20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCCCTATCGAGCAACTCTGCGTTGATACCAC20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCCGAAGAGATGTGTAAGCGCAGCTCACAGA20.11235955056179776No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAAGCTCACAGAGACCAAGTCTC20.11235955056179776No Hit
GCAAAAGTTCTCAGAGATGTGTAACGCAGCTCACAGAGACCAAGTCTCTG20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCACT20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTATCGAGCACAGCTTAG20.11235955056179776No Hit
GCAAAGGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCCGATGAGCAACTCTGCGTTGATACCACTG20.11235955056179776Clontech SMART CDS Primer II A (95% over 21bp)
AACTCCTGTTTAGGCCTTGCGGCCACGATAGGATCACAGAGACCAAGTCT20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAAGCTCACAGAGACCAAGTCTC20.11235955056179776No Hit
GCAAAAGTTCTCAGGGATGTCCCGATCGAGCAACTCTGCGTTGATACCCT20.11235955056179776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)