FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19187
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA669834.90905300463856No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC659834.38786678480221No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG207910.835461510397664No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC5843.04372752384427No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT3892.027414395163392No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT3671.9127534267993955No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG820.427372700265805No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG690.3596184916870798No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG640.3335591806952624No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC610.317923594100172No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC360.1876270391410851No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC330.17199145254599468No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG330.17199145254599468No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG310.16156772814926773No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.14072027935581383No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT260.13550841715745035No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT250.13029655495908687No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC250.13029655495908687No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC230.11987283056235994No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.11466096836399646No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA220.11466096836399646No Hit
AGCACTTGAAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA220.11466096836399646No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC220.11466096836399646No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT210.10944910616563297No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC210.10944910616563297No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.10423724396726951No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)