FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21199
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC1005147.412613802537855No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA662331.242039718854663No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC4842.283126562573706No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT4161.962356714939384No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT2881.3585546488041889No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG2241.0566536157365913No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC910.42926553139299023No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAAGTGGTTCGCAGAAGCAGCA900.424548327751309No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG740.34907306948440964No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG700.3302042549176848No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG680.32076984763432237No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC630.2971838294259163No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC540.2547289966507854No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT540.2547289966507854No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC520.245294589367423No Hit
AGCACTTGAAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA460.21699136751733572No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC440.2075569602339733No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC440.2075569602339733No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.19340534930892966No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.18397094202556724No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC380.17925373838388603No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.17925373838388603No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC350.1651021274588424No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC330.15566772017547997No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC320.15095051653379876No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCTCACAGAGAC310.14623331289211755No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC300.14151610925043634No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT290.13679890560875513No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.13208170196707392No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC270.1273644983253927No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC250.11793009104203028No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC230.10849568375866786No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTT230.10849568375866786No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC230.10849568375866786No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)