FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20157
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC791239.25187279853153No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT515625.579203254452548No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA9604.762613484149427No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT7913.9241950687106217No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA5602.778191199087166No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC4792.376345686362058No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT3241.6073820509004317No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT2331.1559259810487672No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA2311.1460038696234558No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1870.927717418266607No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT1680.8334573597261496No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT780.386962345587141No Hit
TGCGGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC670.33239073274792874No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA460.22820856278216003No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC450.2232475070695044No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT380.18852011708091482No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC360.17859800565560352No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT340.16867589423029222No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC320.1587537828049809No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.15379272709232525No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT300.1488316713796696No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC300.1488316713796696No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.13394850424170263No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT260.12898744852904698No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.12402639281639133No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC250.12402639281639133No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.11906533710373567No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.11906533710373567No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.11410428139108002No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.11410428139108002No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.11410428139108002No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC230.11410428139108002No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.10914322567842437No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC210.1041821699657687No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT210.1041821699657687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)