FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971
Total Bases140.7 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT34335.32440782698249No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT25926.6735324407827No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA14214.62409886714727No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT171.7507723995880538No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA151.544799176107106No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTA111.132852729145211No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC101.0298661174047374No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCCCATGTTGTA80.8238928939237898No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCCCATGTTGTAGAACCATGTC80.8238928939237898No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG60.6179196704428425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCCCTGAGAACTTTTGCCATGTTGTAGAACCATGTC50.5149330587023687No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG50.5149330587023687No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCCCATGTTGTA40.4119464469618949No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCCCATGTTGTAGAACCATGTC40.4119464469618949No Hit
TCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCAT30.30895983522142123No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG30.30895983522142123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTGAGAACTTTGCCCATGTTGTAGAACCATGTC30.30895983522142123No Hit
TCCTATCAGTGGCCGCAGGCCTAAACAGGAGTTCCATGTTGTAGAACCAT30.30895983522142123No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC30.30895983522142123No Hit
GCTGCGCTTACACATCGCTGAGAACTTTTGCCCATGTTGTAGAACCATGT30.30895983522142123No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGACCATGTC20.20597322348094746No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTCCCATGTTGTAGAACCATGTC20.20597322348094746No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCCCATGTTGTAGAACCATGTC20.20597322348094746No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCATGTTGTAGAACCATGTC20.20597322348094746No Hit
GCTGCGCTTACACATTCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT20.20597322348094746No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGAGTTCCATGTTGTAGAACCAT20.20597322348094746No Hit
GCTGCGCTTACACATCTCTTACATCCCTGAGAACTTTTGCCCATGTTGTA20.20597322348094746No Hit
GCTGCGCTTAACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC20.20597322348094746No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC20.20597322348094746No Hit
GCTGCGCTTACACATCCCTGAGACTTTTGCCCATGTTGTAGAACCATGTC20.20597322348094746No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCCCATGTTGTAGAACCATGTC20.20597322348094746No Hit
TCCTATCAGTGGCCGCAAGGCCTAACAGGAGTTCCATGTTGTAGAACCAT20.20597322348094746No Hit
GCTGCGCTTACACATCTCTGAGCCCACGAGACCCCATGTTGTAGAACCAT20.20597322348094746No Hit
GCTGCGCCTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTAGTAGAACAA10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCATGTTGTAGAACCAATGT10.10298661174047373No Hit
GCTGCGTTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTCCATGTTGTAGAACCAT10.10298661174047373No Hit
GCTGCGCTTACACGTCCCTGAGAACTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTCACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTCACATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTAG10.10298661174047373No Hit
GCTGCGCTTACACATCCCGAGAACTTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAGCTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGATTCCATGTTGTAGAACCAT10.10298661174047373No Hit
GCTGCGTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCAGTC10.10298661174047373No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGGACCA10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCTGTC10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGTCGAATGTCGCCGAAGGCTGCGCTTACA10.10298661174047373No Hit
GCTGCGCTTACACATCTCTTCCCAGACCAGTGAATGATTCCTGCCTGGCG10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCCATGTTGTAGAACCATGTCG10.10298661174047373No Hit
GCTGCGTTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTAAACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACTATGT10.10298661174047373No Hit
CGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTCGTC10.10298661174047373No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGGAGAACCA10.10298661174047373No Hit
GCTGCGCTTACACATCTCGGCATGCCTGGGGACGTTTTGCCATGTTGTTT10.10298661174047373No Hit
GCTGCGCTTACACATCCCCGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATCAGTGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCAT10.10298661174047373No Hit
TCCTATCAGTGGCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCAT10.10298661174047373No Hit
TCCTATCAGTGGCAGCAAGGCCTAAACAGGAGTCCATGTTGTAGAACCAT10.10298661174047373No Hit
GCTTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATG10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAAAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GTTGTTTAGAATGGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTC10.10298661174047373No Hit
GCTCGCTTACACATCTACTCGACATCCCTGAGAACTTTTGCCCATGTTGT10.10298661174047373No Hit
GCTGCGCTTACACATCTCTAACATCCCTGAAACTTTTGCCCATGTTGTAG10.10298661174047373No Hit
GTTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TGCTGATCATTGTTGCTTTGGGCAGCCTGTTGATTCAAGTGCTCCATGTT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGGACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACAAATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCAT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTAGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCAT10.10298661174047373No Hit
GCTGCGCTTACACATCTCTTAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGACATCCCTGAGAACTTTTGCCCATGTTGTA10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGCCCACGGACCCCATGTTGTAGAACCATG10.10298661174047373No Hit
GCTGCGCTTACACATCTCTTCGCTTACACATCCCTGAGAACTTTTGCCCA10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACAATGA10.10298661174047373No Hit
GTGCCCATACACATACCAGAGAACATTTGCCCAAGTTTTTAAACCAATTC10.10298661174047373No Hit
GCTGCGCTTACAATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTTACATATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATCAGTGGCGCAAGGCCTAAACAGGAGTCCATGTTGTAGAACCATG10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGACATCCCTAGAACTTTTCCCATGTTGTAGA10.10298661174047373No Hit
GCTGCGCTTACACAACCCTGAGAACTTTTGCCCATCTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGTTACACACCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTCG10.10298661174047373No Hit
TCCTATCAGTGGCCACAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACAATGT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAAGTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
CAACCAAAAGAGAAGGAGGCACTTACACATCCCTGAGAACTTTTGCCCAT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAATTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
TGCTGATCATTGTTGCTTTGGGGCAGCCTGTTGATTCAAGTGCTCCATGT10.10298661174047373No Hit
GCTGCGCTTTCACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTGGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATGAGCTGCTTG10.10298661174047373No Hit
GCTGCGCTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCGATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAGCCATGT10.10298661174047373No Hit
GCTGCGGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATG10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAATTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTTACAAATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTA10.10298661174047373No Hit
TGCTGATCATTGTTGATTCAGTGCTCCATGTTGTAGAACCATGTCGTCAG10.10298661174047373No Hit
GCTGCGCTTACGCATCCCTGAGAACTTTTGCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTCGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGACTTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
TCCTATCGTGGCCCCTAGGCCTAAACAGGAGTTACAAGATGTAGAACCAT10.10298661174047373No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACATGTC10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTTTAGAACCATGT10.10298661174047373No Hit
CCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCTCGAGAACTTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
TCCTTCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCAT10.10298661174047373No Hit
TCCTATCAGTAGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA10.10298661174047373No Hit
GCTGAGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT10.10298661174047373No Hit
TCCTATCAGTGGCCGCATGGCCTAACAGGAGTTCCATGTTGTAGAACCAT10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAAACTTTTGCCCATGTTGTAGAACCATGTC10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCAAGT10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTTGAACCATGT10.10298661174047373No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTACATGTTGTAGAACCA10.10298661174047373No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT10.10298661174047373No Hit
GCTGCGCTTACACATCTCGACATCCCTGAGAACTTTTGCCCATGTTGTAG10.10298661174047373No Hit
GCTTGCGCTTATACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATG10.10298661174047373No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTATGTTGTAGAACCATGT10.10298661174047373No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATTT10.10298661174047373No Hit

[FAIL]Adapter Content

Adapter graph