FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008145970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008145970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29315
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT901330.74535220876684No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC673222.964352720450282No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA501817.11751662971175No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG28219.623059866962306No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC6772.3093979191540166No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT6522.2241173460685655No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT6102.0808459832850077No Hit
CGCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT1400.47757120927852637No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC760.25925294217977146No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC680.23196315879242707No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.20808459832850076No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG580.19785092955824662No Hit
CGCCAGGAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT580.19785092955824662No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTACCATATTCTTTCCG520.17738359201773835No Hit
CGCCAGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT490.16714992324748423No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.15350503155381204No Hit
CGCCGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT430.14668258570697595No Hit
CGCCAGGCAGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT400.13644891693672181No Hit
AGCACTTGAAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA370.12621524816646768No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT340.11598157939621354No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC340.11598157939621354No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC300.10233668770254137No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)