FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253
Total Bases181.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT46537.110933758978454No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCT30724.501197126895452No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTC19315.403032721468476No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGATCACAGAGACCAAGTC161.2769353551476457No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAACATGGGCA141.1173184357541899No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG110.8778930566640064No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACAAAGTCTCT100.7980845969672785No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT90.7182761372705506No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACAAAGTCTCT90.7182761372705506No Hit
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCTCACAGAGAC80.6384676775738228No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG60.4788507581803671No Hit
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG50.39904229848363926No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA40.3192338387869114No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.3192338387869114No Hit
CAAAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.3192338387869114No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG40.3192338387869114No Hit
AACTCCTGTTTAGGCCTCTCTCCTCATTCTAAACAACTCACAGAGACCAA40.3192338387869114No Hit
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACAACATGGG30.23942537909018355No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG30.23942537909018355No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCTCACAGAGAC30.23942537909018355No Hit
CAAAAGTTCTCAGAGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.23942537909018355No Hit
GCAAAGTTCTCAGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTGC30.23942537909018355No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAACTCACAGAGACCAAGTCTCT30.23942537909018355No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCACTCACAGAGACCAAGTCTCTG30.23942537909018355No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCACTCACAGAGACCAAGTCTCTG30.23942537909018355No Hit
GCAAAAGTTCTAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG30.23942537909018355No Hit
AACTCCTGTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTCT30.23942537909018355No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAACATGGCGC20.1596169193934557No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAACATGGCGA20.1596169193934557No Hit
GCAAAAGTTCTCAGGGTGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTG20.1596169193934557No Hit
AATCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTCT20.1596169193934557No Hit
CGCCAGGCAGGAATCACACTGACGACATGGTTCTACAACATGGGCAAAAG20.1596169193934557No Hit
ACTCCTGTTTAGGCCTTGCGGCCACTGATAGGATCACAGAGACCAAGTCT20.1596169193934557No Hit
CGCCAGGCAGGAATCATTCCACTGACGACATGGTTCTACAACATGGGCAA20.1596169193934557No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGATCACAGAGACCAAGTCT20.1596169193934557No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCTTTCTGGAAAACTG20.1596169193934557No Hit
AACTCCTGTTTAGGCCTTTCTCCTCATTCTAAACAACTCACAGAGACCAA20.1596169193934557No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAACATAGGCA20.1596169193934557No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCCCATGTACTCTG20.1596169193934557No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCTCACAGAGAC20.1596169193934557No Hit
GCAAAAGTTCTCAGGGATGTGTAACGCAGCTCACAGAGACCAAGTCTCTG20.1596169193934557No Hit
CGCCAGGCAGGAATCATACTGACGACATGGTTCTACAACATGGGCAAAAG20.1596169193934557No Hit
GAAGTTCTCAGGGATGTGTAAGCGCAGCTCACAGAGACCAAGTCTCTGCT20.1596169193934557No Hit
GCAAAAGTTCTCAGGGATGTGTAAACGCAGCTCACAGAGACCAAGTCTCT20.1596169193934557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)