FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253
Total Bases181.6 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT49039.10614525139665No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT32525.937749401436548No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCA19515.562649640861931No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTCCATGTTGTAGAACCA171.3567438148443736No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG110.8778930566640064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCCCTGAGAACTTTGCCCATGTTGTAGAACCATGTC100.7980845969672785No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG80.6384676775738228Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCGCTTACACATCTCTAACATCCCTGAGAACTTTTGCCCATGTTGTA80.6384676775738228No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCCCATGTTGTAGAACCATGTC60.4788507581803671No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCCCATGTTGTAGAACCATGTC50.39904229848363926No Hit
GTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC40.3192338387869114No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCATGTTGTAGAACCATGTC40.3192338387869114No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC40.3192338387869114No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCCCATGTTGTAGAACCATGTC40.3192338387869114No Hit
TCCTATCAGTGGCCGCAAGGCCTAACAGGAGTTCCATGTTGTAGAACCAT40.3192338387869114No Hit
GTTGTTTAGAATGAGGAGAGAGGCCTAAACAGGAGTTCCATGTTGTAGAA40.3192338387869114No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCCCATGTTGTA30.23942537909018355No Hit
GCTGCGCTTACACATCCTGAGAACTTTGCCCATGTTGTAGAACCATGTCG30.23942537909018355No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCCCATGTTGTAGAACCATGTC30.23942537909018355No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCATGTTGTAGAACCATGTC30.23942537909018355No Hit
GTTGCGCTTACACATCTCTGAGAACTTTTGCCCATGTTGTAGAACCATGT30.23942537909018355No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC30.23942537909018355No Hit
TCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTCCATGTTGTAGAACCAT20.1596169193934557No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTCCATGTTGTAGAACCAT20.1596169193934557No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGATTCCATGTTGTAGAACCAT20.1596169193934557No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGTGCAGGCTCGATAAGCTGCTTG20.1596169193934557No Hit
GCTGCGTTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGT20.1596169193934557No Hit
GCTGCGCTTACACATCCCTGAGAACTTCCCATGTTGTAGAACCATGTCGT20.1596169193934557No Hit
GCTTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATG20.1596169193934557No Hit
GCTGCGCTTACACATCTCTTCTAAGCTGTGCAGGCTCGATGAGCAGGTCG20.1596169193934557No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG20.1596169193934557No Hit
GCTGCGCTTACACACCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC20.1596169193934557No Hit
GCTGCGCTTACACATCGCTGAGAACTTTTGCCCATGTTGTAGAACCATGT20.1596169193934557No Hit
GTTGTTTAGAATGAGGAGAAAGGCCTAAACAGGAGTTCCATGTTGTAGAA20.1596169193934557No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCCCATGTTGTA20.1596169193934557No Hit
GCTGCGTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCATGTC20.1596169193934557No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCAGTC20.1596169193934557No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTATGTTGTAGAACCATGT20.1596169193934557No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCCATGTTGTAGAACCCTGT20.1596169193934557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)