FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24900
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC895135.94779116465863No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT793131.85140562248996No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC9563.8393574297188753No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT8673.4819277108433737No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT6172.477911646586345No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA4371.7550200803212852No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT4251.706827309236948No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT4241.7028112449799195No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA3491.4016064257028111No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT2631.0562248995983936No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1440.5783132530120482No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC1320.5301204819277108No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT1010.4056224899598394No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT890.357429718875502No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT750.30120481927710846No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT670.26907630522088355No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC520.20883534136546186No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT340.13654618473895583No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT340.13654618473895583No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT320.1285140562248996No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC310.1244979919678715No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.11646586345381525No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.11646586345381525No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC290.11646586345381525No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT280.11244979919678715No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT280.11244979919678715No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.10441767068273093No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.1004016064257028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)