FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22606
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC1099948.65522427674069No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA356215.756878704768646No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG285912.647084844731488No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT9704.290896222241883No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT7593.3575157037954524No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT4281.8933026630098204No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGACTGCATAGACCAAGTC3091.366893745023445No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC580.2565690524639476No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC550.24329823940546758No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC480.2123330089356808No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.19906219587720073No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.1902149871715474No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC420.18579138281872068No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC360.1592497567017606No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC350.1548261523489339No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGACTGCATAGACCAAGTC320.14155533929045389No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.14155533929045389No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC290.1282845262319738No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT290.1282845262319738No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC290.1282845262319738No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.12386092187914714No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC280.12386092187914714No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC270.11943731752632045No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.11943731752632045No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC260.11501371317349376No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC250.11059010882066708No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC240.1061665044678404No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.1061665044678404No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC230.1017429001150137No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)