FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22534
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC858038.075796574065855No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT796135.32883642495784No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT9294.122659092926244No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT7013.110854708440579No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA3261.4467027602733646No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA2110.9363628295020857No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT1820.8076684121771545No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTACATCCCTGAGAACTTT1140.5059021922428331No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT940.417147421673915No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT730.323954912576551No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT630.27957752729209195No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT470.2085737108369575No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC400.17750954113783618No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC370.16419632555249844No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.15975858702405255No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT350.15532084849560662No Hit
GCTGCGCTTACACATCTATCCCCTCATCAACACGCCATCCCCTCATCAAC330.14644537143871483No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT320.14200763291026894No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCGACATCCCTGAGAACTTT310.13756989438182302No Hit
GCTGCGCTTACACATCTCTTCCCTCATCAACACGCCATCCCCTCATCAAC300.13313215585337712No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.13313215585337712No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC280.12425667879648532No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT280.12425667879648532No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG280.12425667879648532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.11981894026803941No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.11981894026803941No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT250.1109434632111476No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT250.1109434632111476No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG240.10650572468270171No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT240.10650572468270171No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT230.10206798615425577No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.10206798615425577No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTCCCATGTACTCTGCGTT230.10206798615425577No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT230.10206798615425577No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.10206798615425577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)