FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12861
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG611347.531296166705545No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA342126.599797838426248No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT5524.292045719617448No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGACTGCATAGACCAAGTC3342.5969986781743253No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC3322.5814477878858564No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT2812.184900085529897No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT690.536505714952181No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT650.505403934375243No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT570.44320037322136696No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT540.4198740377886634No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG500.38877225721172537No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.34989503149055284No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGACTGCATAGACCAAGTC390.30324236062514576No Hit
AACTCCTGTTTAGGCCTTGTAGAATTTGGGACTCTGCCATATTCTTTCCG370.28769147033667675No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCCGCCATATTCTTTCCG330.2565896897597387No Hit
AATCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT280.2177124640385662No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCCTTCCG240.18661068346162818No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGGCTCTGCCATATTCTTTCCG230.17883523831739367No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTCCCG210.16328434802892466No Hit
AACTCCTGTTTAGGCCTTGCGGAATTTGGGACTCTGCCATATTCTTTCCG190.14773345774045563No Hit
AACTCCTTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT180.13995801259622112No Hit
AACTCCTGTTTGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT180.13995801259622112No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG180.13995801259622112No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG170.13218256745198662No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATACTCTTTCCG170.13218256745198662No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATGTTCTTTCCG170.13218256745198662No Hit
AACTCCTGTTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCC160.12440712230775211No Hit
AACTCCGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT150.1166316771635176No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATCCTTTCCG150.1166316771635176No Hit
AACCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT150.1166316771635176No Hit
AACTCCTGTTTAGGCCTCTCTCCTCATTCTAAACAACCTGCATAGACCAA150.1166316771635176No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA140.1088562320192831No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCACATTCTTTCCG130.1010807868750486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)