FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21191
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC885341.777169553112174No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT780936.85054976169128No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT5802.7370109952338257No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA5262.4821858336086073No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT4812.269831532254259No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA2591.2222169789061395No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1050.49549336982681325No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT730.3444858666414987No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT530.2501061771506772No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT430.20291633240526635No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC410.19347836345618424No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC380.179321410032561No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT350.16516445660893775No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC340.16044547213439667No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT330.15572648765985558No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.15572648765985558No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC320.15100750318531453No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT320.15100750318531453No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT320.15100750318531453No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT310.14628851871077345No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.14156953423623236No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT300.14156953423623236No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.14156953423623236No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.13685054976169128No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC280.1321315652871502No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.1321315652871502No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.12741258081260914No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC260.12269359633806805No Hit
TGGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT240.1132556273889859No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT240.1132556273889859No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC230.10853664291444481No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.10853664291444481No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT220.10381765843990372No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT220.10381765843990372No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)