FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939
Total Bases136.1 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT51955.27156549520767No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT23625.133120340788075No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA535.644302449414271No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT101.0649627263045793No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCGAATCCTGTAGAACCATGTC80.8519701810436635No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.7454739084132056No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTGAATCCTGTAGAACCA50.5324813631522897No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC40.42598509052183176No Hit
GCTGCGTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC30.3194888178913738No Hit
GCTGCGCTTACACATCCCTGAGACTTTTGCGAATCCTGTAGAACCATGTC30.3194888178913738No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACATGTC30.3194888178913738No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCGAATCCTGTAGAACCATGTC30.3194888178913738No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCGAATCCTGTAGAACCATGTC20.21299254526091588No Hit
GCTGCGCTTACACATCCCGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.21299254526091588No Hit
GCTGCGCTTACACATCGCTGAGAACTTTTGCGAATCCTGTAGAACCATGT20.21299254526091588No Hit
GTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.21299254526091588No Hit
GCTGCGCTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.21299254526091588No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGGAATCCTGTAGAACCATGTC20.21299254526091588No Hit
GCTGCGCTTACACATCTCTTACATCCCTGAGAACTTTTGCGAATCCTGTA20.21299254526091588No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT20.21299254526091588No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCAAGT20.21299254526091588No Hit
GCTGCGCTTACACATCCCGAGAACTTTTCGAATCCTGTAGAACCATGTCG10.10649627263045794No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
GCTGCGCTTACACATCTTTGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
GCTGGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
CAACCAAAAGAAAGGAGGCGGTAGCAGAGACTTGGTCTATGCAGGCTGCG10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
TCCTATCAGTGGCCTAAACAGGAGTTGAATCCTGTAGAACCATGTCGTCA10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCCTGT10.10649627263045794No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAGAAGATCAAGTCAAAT10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCAGTC10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCGTGT10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGAGACTTGCGAATCCTGTAGAACCATGTCGT10.10649627263045794No Hit
CTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
GCCGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
TGCTGAATCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGCCG10.10649627263045794Illumina Single End PCR Primer 1 (95% over 24bp)
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG10.10649627263045794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCAT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCATGTAGAAAAATGT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACTATGT10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACATGTC10.10649627263045794No Hit
GCTGCGCTTACACTTCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGAGACCTTTTGCGAATCCTGTAGCACCATGT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCGAATCCTGTAGAACCATG10.10649627263045794No Hit
GCTGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTCG10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATGAGCTGCTTG10.10649627263045794No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTGCGAATCCTGTAGAACCATGTCG10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGCATCCTGTAGAACCATGT10.10649627263045794No Hit
GCTGTGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
GATGTTTAGAATGAGGAGAGAGGCCTAAACAGGAGTTGAATACTGTAGAA10.10649627263045794No Hit
GCTGCGCTTAACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCTAATCCTGTAGAACCATGT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAGCTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
GCTGCGCTTAAACATCTCTGAGAACTTTTGCGAATCCTGTAGAAAAATGT10.10649627263045794No Hit
TGCTGATCATTGTTGCTTTGCGGGTGAGGAAAAGTCTGGAGTATTTCCTC10.10649627263045794No Hit
GCGGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTAGAAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTTGCGAATCCTGTAGAACCATG10.10649627263045794No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGCACCATGT10.10649627263045794No Hit
GCTGCGCTTACACATTCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
CTGCGCTTACACATCCCTGAGAACTTTTTCGAATACTGTAGAACCAAGTC10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTGAATCCTGTAGAACCAT10.10649627263045794No Hit
GCTGCGCTTACACATCCCCGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
GTTGTTTAGAATGAGGAGAGAGGCCTAAACAGGAGTTGAATCCTGTAGAA10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGAGAATCCTGTAGAACCATGT10.10649627263045794No Hit
TCCTTCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCAT10.10649627263045794No Hit
GTTGTTTAGAATGAGGAGAGAGGCGAAAAAAAAAAAATCAAGTCAAATTT10.10649627263045794No Hit
GTTGTTTAGAATGGGAGAGAGGCCTAAACAGGAGTTGAATCCTGTAGAAC10.10649627263045794No Hit
GCTGCGCTTACGCATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
GTTGTTTAGAATGAGGAGAGGGGCAAAAAAAAAATCAAGTCAAATTTCTT10.10649627263045794No Hit
TCCTATCAGTGGCCGCAAGGCCTAACAGGAGTTGAATCCTGTAGAACCAT10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGACTTTGCGAATCCTGTAGAACCATGTCG10.10649627263045794No Hit
CAACCAAAAGAGAATCAGTCACAACTTTTGCGAAACCTGTAGAAAGAAGT10.10649627263045794No Hit
GCTCGCTTATACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
TCCTATCAGTGGCCGCAAGGCCAAACAGGAGTTGAATCCTGTAGAACCAT10.10649627263045794No Hit
GCTGCGCTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAAGAACCATG10.10649627263045794No Hit
TGATGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTACCTC10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGAGAACGTTTGCGAATCCTGTAGAACCATGT10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGACCATGTC10.10649627263045794No Hit
CTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC10.10649627263045794No Hit
GCTGCGCTTACACATCTCTGANNNCNNTTGCGAATCCNNNNNNNNCATNN10.10649627263045794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)