FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20667
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1219158.98775826196352No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT230311.143368655344268No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT10625.1386267963419945No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT5702.758020031934969No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT5462.641892872695602No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC2921.4128804374122998No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA1820.8806309575652005No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT1680.8128901146755697No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1490.7209561136110707No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA1110.5370881114820728No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT920.4451541104175739No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC570.2758020031934969No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC480.2322543184787342No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC460.22257705520878696No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC420.20322252866889243No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT420.20322252866889243No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC390.18870663376397154No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC380.18386800212899793No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT370.1790293704940243No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC350.16935210722407704No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.15967484395412979No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.15967484395412979No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC320.15483621231915615No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG310.1499975806841825No Hit
TGGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT280.13548168577926162No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC270.130643054144288No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC260.12580442250931437No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC250.12096579087434074No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCGAATCCTGTAGAACCATGTC240.1161271592393671No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.1161271592393671No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTGAATCCTGTAGAACCA230.11128852760439348No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT230.11128852760439348No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT230.11128852760439348No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.11128852760439348No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC230.11128852760439348No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC220.10644989596941985No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC210.10161126433444621No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTACATCCCTGAGAACTTT210.10161126433444621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)