FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15280
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA935761.23691099476439No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT10046.570680628272251No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT9206.020942408376963No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG4432.899214659685864No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAA3622.369109947643979No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGACTGCATAGACCAAGTC1490.9751308900523561No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT1420.9293193717277487No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1180.7722513089005236No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1180.7722513089005236No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG690.4515706806282722No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC590.3861256544502618No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.3468586387434555No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT530.3468586387434555Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG510.33376963350785344No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGAGATGTGTAAGCGC500.32722513089005234No Hit
GCAAAAGTTCTCAGGGATGTCCAGGCAAAAGTTCTCAGGGATGTCCAGGC450.29450261780104714No Hit
GCAAAAGTTCTCAGGGATGTAGGCAAAAGTTCTCAGGGATGTAGGCAAAA410.2683246073298429No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA340.22251308900523561No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG320.20942408376963353No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.18979057591623036No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.18324607329842932No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATGAGGGGATGGCGTGTTG270.17670157068062828No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCCCATGTACTCTG260.1701570680628272No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCCTGCATAGAC250.16361256544502617No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA230.15052356020942406No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGCTCGCAGAAGCAGCA220.14397905759162302No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG210.13743455497382198No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA210.13743455497382198No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA200.13089005235602094No Hit
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG200.13089005235602094No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCTG200.13089005235602094No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCCTGCATAGAC200.13089005235602094No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCCTGCATAGACAAAGTCTCT200.13089005235602094No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA190.12434554973821989No Hit
GCAAAAGTTCTCAGGGATGTCCCTAAGCTGCTTGGGGATGACCAGGCAAA190.12434554973821989No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA180.11780104712041885No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG180.11780104712041885No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA170.11125654450261781No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA170.11125654450261781No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACAAAGTCTCT160.10471204188481677No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAGGCAGCA160.10471204188481677No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGCGGTTCGCAGAAGCAGCA160.10471204188481677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)