FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10411
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG625360.0614734415522No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA6426.166554605705503No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT5945.70550379406397No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT4854.658534242627989No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC4234.063010277591009No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGACTGCATAGACCAAGTC2782.6702526174238788No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGGGATGTAGGCAAAA990.9509172990106618No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT620.5955239650369801No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT580.5571030640668524No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT480.461050811641533No Hit
AACTCCTGTTTAGGCCTTGTAGAATTTGGGACTCTGCCATATTCTTTCCG360.34578810873114973No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT250.24013063106329843No Hit
AACTCCTTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT240.2305254058207665No Hit
AACTCCGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT240.2305254058207665No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGGCTCTGCCATATTCTTTCCG230.22092018057823454No Hit
AACTCCTGTTTGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT230.22092018057823454No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGACTGCATAGACCAAGTC220.21131495533570263No Hit
AATCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT220.21131495533570263No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCCTTCCG200.19210450485063874No Hit
AACTCCTGTTTAGGCCTCTCTCCTCATTCTAAACAACCTGCATAGACCAA180.17289405436557487No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCTG170.16328882912304293No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCCTGCATAGAC160.153683603880511No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACCCTGCCATATTCTTTCCG160.153683603880511No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATCCTTTCCG150.14407837863797907No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCCGCCATATTCTTTCCG140.13447315339544713No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATACTCTTTCCG130.1248679281529152No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA120.11526270291038325No Hit
AACCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT120.11526270291038325No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCACATTCTTTCCG120.11526270291038325No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTCTCCG110.10565747766785132No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCCTGCATAGAC110.10565747766785132No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCGTATTCTTTCCG110.10565747766785132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)