FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10411
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT209520.122946883104408No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT147814.196522908462203No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT6215.964844875612333No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT6105.859187397944482No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC5755.523004514455864No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT5605.378926135817885No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT5024.821823071751032No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC4184.01498415137835No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA2772.660647392181347No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT1841.7673614446258763No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT940.902891172798002No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAAATCAAGTCAAA850.8164441456152147No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTAAAATT780.7492075689174912No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA450.4322351359139372No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAAATAAAGTCAAA410.3938142349438094No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTAAAAT370.35539333397368167No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTACATCCCTGAGAACTTT350.3361828834886178No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCATGTCAAAT330.31697243300355393No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT310.29776198251849006No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAATTT290.2785515320334262No Hit
GTTGTTTAGAATGAGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATTT250.24013063106329843No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTGAATCCTGTAGAACCA220.21131495533570263No Hit
GTTGTTTAGATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATTT220.21131495533570263No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAAAATCAAGTCAA220.21131495533570263No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG220.21131495533570263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATAAAGTCAAATTT200.19210450485063874No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCATGTCAAATT200.19210450485063874No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGGCAAAT190.1824992796081068No Hit
GTTGTTTAGATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTTC180.17289405436557487No Hit
GTTGTTTAGAATGAGGAGAGAGGCCTAAACAGGAGTTGAATCCTGTAGAA180.17289405436557487No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATAAAGTCAAAT160.153683603880511No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCGAATCCTGTAGAACCATGTC160.153683603880511No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAACAAGTCAAATTT160.153683603880511No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATAAAGTAAAATT160.153683603880511No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCGAATCCTGTA150.14407837863797907No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATAAAGTCAAATT150.14407837863797907No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTA150.14407837863797907No Hit
GTTGTTTAGAATGAGGAGAGAGCAAAAAAAAAAAAAATCAAGTCAAATTT140.13447315339544713No Hit
GTTGTTTAGAATGGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATTT140.13447315339544713No Hit
GTTGTTTAGAATAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATTT140.13447315339544713No Hit
GTTGTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATTT140.13447315339544713No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTAAAATTT120.11526270291038325No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATAAAGTCAAATTTC110.10565747766785132No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATAAAGTAAAAT110.10565747766785132No Hit
GCTGCGCTTACACATCTCGGACATCCCTGAGAACTTTTGCGAATCCTGTA110.10565747766785132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)