FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19339
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length144-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1004651.946843166658056No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT15437.978695899477739No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT10865.615595428926005No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC8734.514194115517865No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT7013.6247996276953307No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT6093.149076994673975No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT3171.6391747246496717No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT2691.3909716117689643No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA2551.318579037178758No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1240.6411913749418274No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1180.610165985831739No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT570.29474119654584No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAAATCAAGTCAAA500.25854490925073687No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC460.23786131651067788No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT400.20683592740058948No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT380.19649413103056002No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT380.19649413103056002No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC380.19649413103056002No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTAAAATT350.1809814364755158No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC330.17063964010548632No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.17063964010548632No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC250.12927245462536843No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTAGAGTATTTCCTC240.1241015564403537No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC240.1241015564403537No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCGAATCCTGTA230.11893065825533894No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.11893065825533894No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC220.1137597600703242No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATAAAGTCAAATTTC220.1137597600703242No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT220.1137597600703242No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.1137597600703242No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCGAATCCTGTAGAACCATGTC210.10858886188530947No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAACAAGTCAAATTT210.10858886188530947No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.10858886188530947No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCATGTCAAAT200.10341796370029474No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAATTTT200.10341796370029474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)