FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23244
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC935040.225434520736535No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA626126.93598347960764No Hit
AACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCG332714.313371192565825No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT6802.925486146962657No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCCTGCATAGACCAAGTCTCT3771.6219239373601788No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGACTGCATAGACCAAGTC3501.5057649285837205No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG850.3656857683703321No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG720.3097573567372225No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC540.23231801755291687No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC440.18929616245052486No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.16778523489932887No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC340.14627430734813285No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC340.14627430734813285No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC330.14197212183789365No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC310.13336775081741525No Hit
AACTCCTGTTTAGGCTTTGCGGCCACTGATAGGACTGCATAGACCAAGTC290.12476337979693684No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.11615900877645843No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.11615900877645843No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.11185682326621924No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC260.11185682326621924No Hit
AACTCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT260.11185682326621924No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC250.10755463775598004No Hit
ACTCCTGTTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT250.10755463775598004No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC250.10755463775598004No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC250.10755463775598004No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC240.10325245224574084No Hit
AACTCCTGTTAGGCCTTGCAGAATTTGGGACTCTGCCATATTCTTTCCGT240.10325245224574084No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)