FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23206
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1290955.62785486512109No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT556823.993794708265103No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT5652.4347151598724466No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT5302.283892096871499No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA3241.3961906403516333No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA2731.1764198914073947No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1360.5860553305179695No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT670.2887184348875291No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC660.28440920451607343No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC570.2456261311729725No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT520.22407997931569423No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA510.21977074894423856No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT450.1939153667155046No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT440.18960613634404896No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT430.1852969059725933No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC420.18098767560113763No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC390.16805998448677067No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC390.16805998448677067No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC380.163750754115315No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT370.15944152374385934No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT350.15082306300094803No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC350.15082306300094803No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT340.14651383262949239No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC340.14651383262949239No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC340.14651383262949239No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT320.13789537188658108No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC300.12927691114366974No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.12496768077221408No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC290.12496768077221408No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.11634922002930277No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC260.11203998965784712No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.11203998965784712No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT250.10773075928639145No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)