FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25064
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1024440.871369294605806No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC972638.80466007022024No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT7863.135971911905522No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT4971.9829237152888604No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA3991.5919246728375358No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA3361.3405681455473986No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT960.3830194701563996No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT760.30322374720714973No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT680.2713054580274497No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT470.1875199489307373No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT460.1835301627832748No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT450.17954037663581232No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC420.16757101819342482No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.16757101819342482No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT360.14363230130864987No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT340.13565272901372485No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC310.12368337057133737No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.12368337057133737No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT310.12368337057133737No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.11969358442387489No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.11969358442387489No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTACATCCCTGAGAACTTT300.11969358442387489No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.11570379827641239No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.11570379827641239No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT280.11171401212894988No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC280.11171401212894988No Hit
GTTGTTTAGAATGAGGAGAGAGGCCTAAACAGGAGTTGAATCCTGTAGAA280.11171401212894988No Hit
TCCTATCAGTGGCCTAAACAGGAGTTGAATCCTGTAGAACCATGTCGTCA270.1077242259814874No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT260.1037344398340249No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT260.1037344398340249No Hit
TCCTATCAGTGGCCGCAAAGAGGGAAGAGGCCCTGGGCTTCACTTACTCT260.1037344398340249No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)