FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16652
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT438126.30915205380735No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT278216.706701897669948No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC182110.93562334854672No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT9145.4888301705500835No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC7004.203699255344703No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAATCAAGTCAAATTTCT5993.5971655056449676No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT5643.3869805428777324No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA3722.2339658899831853No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA3672.203939466730723No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT3342.0057650732644725No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAATCAAGTCAAATTTCTT1620.9728561133797742No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAATCAAGTCAAAT1520.9128032668748499No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTGAATCCTGTAGAACCA400.24021138601969735No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATAAAGTCAAATTTC370.22219553206822001No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAATTTT340.20417967811674273No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTAAAATT330.1981743934662503No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT330.1981743934662503No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAACAAGTCAAATTT310.18616382416526545No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.17415325486428057No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATAAAGTCAAATTT290.17415325486428057No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC260.15613740091280326No Hit
GTTGTTTAGATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTTC250.15013211626231082No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT240.1441268316118184No Hit
GTTGTTTAGAATGAGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTTC240.1441268316118184No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.13211626231083354No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.13211626231083354No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTA220.13211626231083354No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT210.1261109776603411No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAAAATCAAGTCAAA210.1261109776603411No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAATCAAGTCAAATTTCTTA210.1261109776603411No Hit
GTTGTTTAGAATGAGGAGGAGGCAAAAAAAAAAAAATCAAGTCAAATTTC190.11410040835935624No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT190.11410040835935624No Hit
GTTGTTTAGAATGAGGAGAGAGCAAAAAAAAAAAAATCAAGTCAAATTTC180.10809512370886379No Hit
GTTGTTTAGAATGGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTTC180.10809512370886379No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTAAAATTT180.10809512370886379No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT180.10809512370886379No Hit
GTTGTTTAGATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTCT180.10809512370886379No Hit
GTTGTTTAGAATGAGGAGAGAGGCCTAAACAGGAGTTGAATCCTGTAGAA180.10809512370886379No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)