FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22046
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1182853.65145604644833No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT544924.7165018597478No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT6382.893949015694457No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT4922.231697360065318No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA3321.5059421210196862No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA2251.0205933049079197No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1380.6259638936768575No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCAAATTT1120.5080286673319423No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAAATTTC820.37194956001088636No Hit
GCTGCGCTTACACATCTCTTCCACTTCTTTTACTCTGGCATATTTGAGAC610.2766941848861471No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC560.25401433366597115No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC490.22226254195772477No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT490.22226254195772477No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT470.21319060146965438No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.19051075024947836No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC400.18143880976140797No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT380.17236686927333758No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT370.16783089902930237No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT360.16329492878526716No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT350.15875895854123195No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT340.15422298829719677No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC320.14515104780912635No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC290.13154313707702076No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC290.13154313707702076No Hit
TGGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT280.12700716683298557No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT280.12700716683298557No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC280.12700716683298557No Hit
GTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAAATCAAGTCAAATT270.12247119658895039No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC260.11793522634491518No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC250.11339925610087999No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.10886328585684478No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.10886328585684478No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.10432731561280958No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC230.10432731561280958No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)