FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20682
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC1349165.23063533507397No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT17648.529155787641427No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT8774.2404022821777385No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT7653.698868581375109No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT2121.0250459336621216No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA1800.8703220191470844No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1350.6527415143603132No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTACATCCCTGAGAACTTT1110.5366985784740353No Hit
GCTGCGCTTACACATCTCTTCCCTCATCAACACGCCATCCCCTCATCAAC630.30461270670147955No Hit
GTTGTTTAGAATGAGGAGAGAGGCCCTGGGCTTCACTTACTCTTCATTCA630.30461270670147955No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC570.27560197272991005No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC560.27076685040131515No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT550.26593172807272025No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT450.2175805047867711No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCGACATCCCTGAGAACTTT430.20791026012958128No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT420.20307513780098638No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG420.20307513780098638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC370.1788995261580118No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT370.1788995261580118No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT360.17406440382941687No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC350.16922928150082198No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT350.16922928150082198No Hit
GCTGCGCTTACACATCTATCCCCTCATCAACACGCCATCCCCTCATCAAC320.15472391451503723No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC310.1498887921864423No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC280.13538342520065758No Hit
TGGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT270.13054830287206268No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC270.13054830287206268No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT260.12571318054346775No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC250.12087805821487284No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC240.11604293588627793No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC240.11604293588627793No Hit
GCTGCGCTTACACATCTCTCCCCTCATCAACACGCCATCCCCTCATCAAC240.11604293588627793No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCGAATCCTGTA220.1063726912290881No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCGAATCCTGTAGAACCATGTC220.1063726912290881No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTGACATCCCTGAGAACTT220.1063726912290881No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.1063726912290881No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC210.10153756890049319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)