FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515
Total Bases219.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT78051.48514851485149No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGT34522.772277227722775No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGGAGTTGAATCCTGTAGAACCA1097.194719471947194No Hit
GCTGCGCTTACACATCCCTGAGAACTTTGCGAATCCTGTAGAACCATGTC181.188118811881188No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCTACATCCCTGAGAACTTT110.7260726072607261No Hit
GCTGCGCTTACACATCTCTGAGAACTTTGCGAATCCTGTAGAACCATGTC80.528052805280528No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTCGAATCCTGTAGAACCATGTC70.46204620462046203No Hit
CTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC70.46204620462046203No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTACATCCCTGAGAACTTT70.46204620462046203No Hit
TCCTATCAGTGGCCGCAAAGCCTAAACAGGAGTTGAATCCTGTAGAACCA60.39603960396039606No Hit
GTTGTTTAGAATGAGGAGAGAGGCCTAAACAGGAGTTGAATCCTGTAGAA60.39603960396039606No Hit
GCTGCGCTTACACATCCCTGAGACTTTTGCGAATCCTGTAGAACCATGTC50.33003300330033003No Hit
GCTGCGCTTACACATCCCCGAGAACTTTTGCGAATCCTGTAGAACCATGT50.33003300330033003No Hit
GCTGCGCTTACACATCTCTGCTAAGCAGTGGTATCAACGCAGAGTACATG40.264026402640264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC40.264026402640264No Hit
GCTGCGCTTACACATCTCTGACATCCCTGAGAACTTTTGCGAATCCTGTA40.264026402640264No Hit
GCTGCGCTTACACATCCCTGAAACTTTTGCGAATCCTGTAGAACCATGTC30.19801980198019803No Hit
GCTGCGCTTACACATCCCTGAGAACTTTCGAATCCTGTAGAACCATGTCG30.19801980198019803No Hit
GCTGCGCTTACGCATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT30.19801980198019803No Hit
GCTGCGCTTACACATCTCTGAGAATTTTTGCGAATCCTGTAGAACCATGT30.19801980198019803No Hit
GCTGCGTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC30.19801980198019803No Hit
GCTGCGCTTACACATCTCTTCTAAGCAGTGGTATCAACGCAGAGTACATG30.19801980198019803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT30.19801980198019803No Hit
GCTGCGCTTACACATCGCTGAGAACTTTTGCGAATCCTGTAGAACCATGT30.19801980198019803No Hit
GTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC30.19801980198019803No Hit
GTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC30.19801980198019803No Hit
GCTGCGCTTACACATCCCTGGGAACTTTTGCGAATCCTGTAGAACCATGT20.132013201320132No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACATGTC20.132013201320132No Hit
GCTGCGCTTACACATCTCTGAGAACTTTTGCCTGGACATCCCTGAGAACT20.132013201320132No Hit
GCTGCGTTACACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCTGCGCTTACACATCCTTGAGAACTTTTGCGAATCCTGTAGAACCATGT20.132013201320132No Hit
GCAGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT20.132013201320132No Hit
GCTGCGCTTACACATCCCGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCTGCGCTTACACATCTCTGAGACTTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCTGCGCTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCTGCGCTTACACATCCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCCGAATCCTGTAGAACCATG20.132013201320132No Hit
GTTGTTTAGAATGAGGAGAAAGGCCTAAACAGGAGTTGAATCCTGTAGAA20.132013201320132No Hit
GCTGCGCTTACACATCCCTAGAACTTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
TCCTATCAGTGGCCGCAAGGCCTAAACAGAGTTGAATCCTGTAGAACCAT20.132013201320132No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACAATGT20.132013201320132No Hit
GCTGCGCTTACACATCTCTGCTAAGCTGTGCAGGCTCGATAAGCTGCTTG20.132013201320132No Hit
GCTGCGCTTAACATCTCTGAGAACTTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCGGCGCTTACACATCCCTGAGAACTTTTGCGAATCCTGTAGAACCATGT20.132013201320132No Hit
GCTGCGCTTACACATCCCTGAGAATTTTGCGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCTGCGCTTACACATCCCTGAGAACTTTTGGAATCCTGTAGAACCATGTC20.132013201320132No Hit
GCTGCGCTTACACATCTATCCCCTCATCAACACGCCATCCCCTCATCAAC20.132013201320132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)