FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008146970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008146970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15613
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA427727.393838467943382No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA229614.705693972971243No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG226914.53276116057132No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGCTGGATCCAGAA12297.87164542368539No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA9656.180746813552808No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2611.6716838531992568No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1991.2745788765772113Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA1921.2297444437327867No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC1811.1592903349772625No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1611.031191955421764No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC1120.7173509255107924No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT840.5380131941330942No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG580.371485300710946No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG500.3202459488887466No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG460.2946262729776468No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC460.2946262729776468Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT450.2882213539998719Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT450.2882213539998719No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA410.26260167808877216No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT400.25619675911099726No Hit
GTGCTGTCTCTCTTTGATGGAATCCATGCCTGAATCTGCGGTATACAATT380.2433869211554474No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA300.19214756933324792No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT280.1793377313776981Clontech SMART CDS Primer II A (96% over 25bp)
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT270.17293281239992314No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC270.17293281239992314No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT250.1601229744443733No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.1601229744443733No Hit
GCAAAAGTTCTCAGGGATGTCGTGCCTCCTTCTCTTTTGGTTGTTACGCA240.15371805546659834No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGGGCTGGATCCAGAAA230.1473131364888234No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT220.14090821751104848No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.13450329853327356No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGGGCTGGATCCAGAAA210.13450329853327356No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.13450329853327356No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA210.13450329853327356No Hit
GCAAAAGTTCTCAGGGATGTCCGTCTGCATGCAGTTGACCACATCGGGGA210.13450329853327356No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT210.13450329853327356No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAG200.12809837955549863No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC200.12809837955549863No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC200.12809837955549863No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCT190.1216934605777237No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC180.11528854159994877No Hit
GTGCTGTCTCTCTTGGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA180.11528854159994877No Hit
GCAAAAGTTCTCAGGGATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTC180.11528854159994877No Hit
GCAAAAGTTCTCAGGGATGTCCTTAAAGCATAACATTAAGTCTGTGCCTC170.10888362262217383No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGCTGGATCCAGAAA170.10888362262217383No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC160.1024787036443989No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)