FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37556
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1533940.843007775055916No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1160330.89519650655022No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA23716.3132388965811055No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT8102.1567792097134943No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG3170.8440728512088614No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG2830.753541378208542No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA2370.6310576206198742No Hit
CAACCAAAAGAGAAGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1580.42070508041324955No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1580.42070508041324955No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT1430.38076472467781447No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA1410.3754393439130898No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1280.3408243689423794No Hit
CAACCAAAAAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1200.3195228458834807No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1100.2928959420598573Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1090.2902332516774949No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1050.2795824901480456No Hit
CAACCAAAAGGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1010.2689317286185962No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG600.15976142294174034No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA570.15177335179465332No Hit
TCCTATCAGTGGCCGCAAAGAGAGGAGAGGCCCTGGGCTTCACTTACTCT560.14911066141229098No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT560.14911066141229098No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA560.14911066141229098No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG540.1437852806475663No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC520.1384598998828416No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACG510.1357972095004793No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT500.13313451911811694No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC450.11982106720630524No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT410.10917030567685589No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT410.10917030567685589No Hit
CAACCAAAAGAGAAGGGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG400.10650761529449357No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGGCA390.10384492491213122No Hit
CAACCAAAAGAGAAGGAGGCACCACGGGTTTCTGGATCCAGCCCCTGACA390.10384492491213122No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA390.10384492491213122No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGTCCCTGACA380.10118223452976888No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT380.10118223452976888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)