FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3779
Total Bases547.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA148139.19026197406721No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC2386.297962423921673No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1895.001323101349564Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1203.175443238952104No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA992.6197406721354857No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT912.4080444562053454Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG852.2492722942577403No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG852.2492722942577403No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG611.6141836464673196No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA561.4818735115109818No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT531.4024874305371793No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC381.0055570256681663Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG370.9790949986768986No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT370.9790949986768986No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.793860809738026No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC260.6880127017729558No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA230.6086266207991532No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT210.5557025668166181Clontech SMART CDS Primer II A (96% over 25bp)
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.5557025668166181No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA190.5027785128340831No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG160.42339243186028047No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG150.396930404869013No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.396930404869013No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT150.396930404869013No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA140.37046837787774545Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA130.3440063508864779No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC130.3440063508864779No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.31754432389521037No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG110.2910822969039428No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA100.26462026991267534No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT100.26462026991267534Clontech Universal Primer Mix Long (95% over 23bp)
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA80.21169621593014024No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.21169621593014024No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA70.18523418893887272No Hit
GCAAAAGTTCTCAGGGATGTCTGCGTTGATACCACTGCTTCCCATGTACT70.18523418893887272Clontech Universal Primer Mix Long (95% over 24bp)
GTGCTGTCTCTCTTTGATGTACTCTGCGTTGATACCACTGCTTCCCATGT70.18523418893887272Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCCATGTACTCTGCGTTGATACCACTGCTT70.18523418893887272Clontech SMART CDS Primer II A (96% over 26bp)
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.15877216194760518No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA60.15877216194760518Clontech SMART CDS Primer II A (96% over 26bp)
CTCCAAGTACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTGC60.15877216194760518No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT60.15877216194760518No Hit
GGTGTTTGGGATGGAATGGTGGAGCGCTTGTTGTAGTGCGCCAGGCGGGA50.13231013495633767No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC50.13231013495633767No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT50.13231013495633767Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA50.13231013495633767No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTCTC50.13231013495633767No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC50.13231013495633767No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC50.13231013495633767No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATCTGGGACTCTGCGTTGATACCAC50.13231013495633767No Hit
GCAAAAGTTCTCAGGGATGTCCCCCCATGTACTCTGCGTTGATACCACTG40.10584810796507012Clontech SMART CDS Primer II A (95% over 23bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA40.10584810796507012No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG40.10584810796507012No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA40.10584810796507012No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTTACGCAGAC40.10584810796507012No Hit
CTCCAAGTAACGGTGCTGTCTACTGGCTGGTGCAGGGCTCCTGGTGCTGA40.10584810796507012No Hit
CTCCAAGTAACGGTGCTATCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA40.10584810796507012No Hit
CTCCAAGTAATGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA40.10584810796507012No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGATTACGCAGACCAAG40.10584810796507012No Hit
CTCCAAAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAG40.10584810796507012No Hit
CTCCAAGTAACGGTGCTGCCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA40.10584810796507012No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT40.10584810796507012No Hit
GCAAAAGTTCTCAGGGATGTGCGTTGATACCACTGCTTCCCATGTACTCT40.10584810796507012Clontech Universal Primer Mix Long (95% over 21bp)
CTCTAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA40.10584810796507012No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGTTACGCAGACC40.10584810796507012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)