FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10575
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA565353.45626477541371No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA120911.432624113475178No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA4424.17966903073286No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC2602.458628841607565No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG2091.9763593380614657No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2071.9574468085106382Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1391.3144208037825058No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT990.9361702127659575Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC900.851063829787234No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA860.8132387706855793No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC840.7943262411347518No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA620.5862884160756502No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG610.5768321513002365No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC570.5390070921985816No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG440.4160756501182033No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC440.4160756501182033Clontech SMART CDS Primer II A (95% over 24bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.40661938534278963No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG410.3877068557919622No Hit
GGTGTTTGGGATGGAATGGTGATCCATGCAGGTGGTTCGCAGAAGCAGCA410.3877068557919622No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT390.36879432624113473No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA300.28368794326241137No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT250.2364066193853428Clontech SMART CDS Primer II A (96% over 25bp)
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA250.2364066193853428No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.22695035460992907No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.22695035460992907No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.21749408983451538No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA200.18912529550827423No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.1796690307328605No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG190.1796690307328605No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA180.1702127659574468No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA170.1607565011820331No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT150.14184397163120568No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC150.14184397163120568No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA140.13238770685579196No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.12293144208037825No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA130.12293144208037825No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG120.11347517730496454No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.11347517730496454No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.11347517730496454No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAGGCAGCA110.10401891252955082No Hit
GGTGTTTGGGATGGAATGGTGATCCACACAGGTGGTTCGCAGAAGCAGCA110.10401891252955082No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG110.10401891252955082No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA110.10401891252955082No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA110.10401891252955082No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA110.10401891252955082No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA110.10401891252955082No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)