FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16888
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1065163.06845097110374No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA16749.912363808621507No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT11096.56679298910469No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC2061.2198010421601135No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2021.196115585030791Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1931.1428233064898152No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA1170.692799621032686No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC640.37896731406916156No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA590.3493604926575083Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT550.3256750355281857No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT460.2723827569872099No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA430.2546186641402179No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA430.2546186641402179No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT410.2427759355755566No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC410.2427759355755566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT360.21316911416390336No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT350.2072477498815727No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA340.20132638559924204No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA330.1954050213169114No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.17764092846991947No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA290.17171956418758882No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.16579819990525818No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC280.16579819990525818No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.1598768356229275No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA270.1598768356229275No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.14803410705826622No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT230.13619137849360494No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT220.13027001421127427No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT200.11842728564661299No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA200.11842728564661299No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT200.11842728564661299No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT200.11842728564661299No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.11250592136428234No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.11250592136428234No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT190.11250592136428234No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC190.11250592136428234Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT190.11250592136428234No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT190.11250592136428234No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT180.10658455708195168No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT180.10658455708195168No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC170.10066319279962102Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA170.10066319279962102Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT170.10066319279962102No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCATGGGCTTCACTTACTCT170.10066319279962102No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)