FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191
Total Bases752.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA158930.61067231747255No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG106720.55480639568484No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC2564.931612406087459No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA2084.006935079946061No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC1853.56386052783664No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1252.408013870159892Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC711.367751878250819Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT641.2329031015218648Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG310.5971874397996533No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC260.5008668849932576No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA230.44307455210942015Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.44307455210942015No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT220.423810441148141Clontech SMART CDS Primer II A (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA210.40454633018686187No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.3852822192255827No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT200.3852822192255827No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG190.3660181082643036No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA170.32748988634174536Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA170.32748988634174536No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT160.3082257753804662No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG150.2889616644191871No Hit
GCAAAAGTTCTCAGGGATGTCCTCCCTGGGCCAGCATCCTCTGTACCAAC140.2696975534579079No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT130.2504334424966288No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA130.2504334424966288No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA120.23116933153534963No Hit
GCAAAAGTTCTCAGGGATGTGGTGCCTCCTTCTCTTTTGGTTGTTACGCA110.2119052205740705No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.2119052205740705No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCT110.2119052205740705No Hit
GCAAAAGTTCTCAGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA100.19264110961279135No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAC100.19264110961279135No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTTCCGAAAACCAACAAAATAGAAC100.19264110961279135No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC100.19264110961279135No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT90.17337699865151224No Hit
GCAAAAGTTCTCAGGGATGTGGCGACGGAAAAGCTCAGAGCCGCCTTCAG90.17337699865151224No Hit
GCAAAAGTTCTCAGGGATGTCGGAGATGTACTGGGGGCTGTATAGCCCAC90.17337699865151224No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA80.1541128876902331No Hit
GTGCTGTCTCTCTTTGATGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC80.1541128876902331No Hit
GTGCTGTCTCTCTTTGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC70.13484877672895396No Hit
GCAAAAGTTCTCAGGGATGTCCATGAGGTTTGTTTTTAATTTTATTTTCT70.13484877672895396No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT70.13484877672895396No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTCATGGTTTAG70.13484877672895396No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.13484877672895396No Hit
GCAAAAGTTCTCAGGGATGTCCTGATCCAACATCGAGGTCATCCCCAAGC60.11558466576767482No Hit
GCAAAAGTTCTCAGGGATGTCCCCCCATGTACTCTGCGTTGATACCACTG60.11558466576767482Clontech SMART CDS Primer II A (95% over 23bp)
GTGCTGTCTCTCTTTGATGGACCCCATGTACTCTGCGTTGATACCACTGC60.11558466576767482Clontech SMART CDS Primer II A (95% over 24bp)
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT60.11558466576767482No Hit
GTGCTGTCTCTCTTTGATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC60.11558466576767482No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGTTACGCAGACC60.11558466576767482No Hit
GCAAAAGTTCTCAGGGATGTCCACTGATTCTGCCCATATCCCCACCCACT60.11558466576767482No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT60.11558466576767482No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG60.11558466576767482No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)