FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191
Total Bases751.8 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT159330.68772876131767No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA104320.09246773261414No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4348.360624157195145Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA2134.103255634752456No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA1853.56386052783664No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1292.485070314005009Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1112.1383163167019843Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA731.406280100173377Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC531.0209978809477942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC430.8283567713350029Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA350.6742438836447698No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC310.5971874397996533No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG190.3660181082643036No Hit
GCATCAATCATCACAGGGTTGGTACAGAGGATGCTGGCCCAGGGAGGACA190.3660181082643036No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC190.3660181082643036No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC150.2889616644191871No Hit
CAACCAAAAGAGAAGGAGGCACAGGGGAGGGAGTGTTCATTCAGCATCCC140.2696975534579079No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG140.2696975534579079No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT130.2504334424966288No Hit
CAACCAAAAGAGAAGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG130.2504334424966288No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGTGGT120.23116933153534963Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT120.23116933153534963No Hit
CAACCAAAAGAGAAGGAGGCACCACATCCCTGAGAACTTTTGCCCTCTAT110.2119052205740705No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC100.19264110961279135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT90.17337699865151224No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA90.17337699865151224No Hit
CAACCAAAAGAAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG80.1541128876902331No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.1541128876902331No Hit
CAACCAAAAGAGAAGGAGGCACTGAGGGGCCAGAGCAGCCCCCACCACCT80.1541128876902331No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT80.1541128876902331No Hit
GCATCAATCATCACAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGT70.13484877672895396No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG70.13484877672895396No Hit
CAACCAAAAGAGAAGGAGGCACAGTTTGGGGAATGGGAGTCAGAGTGGGT70.13484877672895396No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGCT70.13484877672895396Clontech Universal Primer Mix Long (95% over 22bp)
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT70.13484877672895396No Hit
CAACCAAAAGAGAAGGGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG70.13484877672895396No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCCCTCTATGTAG60.11558466576767482No Hit
GCATCAATCATCACAGGGCCTGGATCCTAAGTCCGGCAGCGGTATGGTGA60.11558466576767482No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT60.11558466576767482No Hit
CAACCAAAAGAGAAGGAGGCACATGACAGTGAGGGCCAGTTGAAGTGACA60.11558466576767482No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)