FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002
Total Bases435 kbp
Sequences flagged as poor quality0
Sequence length135-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA66322.085276482345105No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA39413.124583610926049No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA32910.95936042638241No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC1414.696868754163891No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1123.7308461025982673No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1063.530979347101932No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1023.397734843437708Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC862.8647568287808127No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC371.2325116588940705No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG361.1992005329780147No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA311.0326449033977347No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC260.866089273817455Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA240.7994670219853431No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA210.6995336442371752No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA210.6995336442371752No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC190.6329113924050633No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA160.5329780146568954No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTTGGTGGTTCGCAGAAGC160.5329780146568954No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT150.4996668887408394No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA140.4663557628247834No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT130.4330446369087275Clontech Universal Primer Mix Long (95% over 23bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT120.39973351099267157Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG120.39973351099267157No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT120.39973351099267157No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.3331112591605596No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC100.3331112591605596No Hit
GTGCTGTCTCTCTTTGATGTACTCTGCGTTGATACCACTGCTTCCCATGT90.29980013324450366Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC90.29980013324450366No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC90.29980013324450366No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC80.2664890073284477No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT80.2664890073284477Clontech SMART CDS Primer II A (96% over 25bp)
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.2664890073284477No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA80.2664890073284477No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGATTACGCAGACCAAG80.2664890073284477No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG80.2664890073284477No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC70.2331778814123917No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT70.2331778814123917No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT70.2331778814123917Clontech Universal Primer Mix Long (96% over 26bp)
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC70.2331778814123917No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG70.2331778814123917No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC60.19986675549633579No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCAAATCCCATGTACTCT60.19986675549633579No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA50.1665556295802798No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG50.1665556295802798No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.1665556295802798No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.1665556295802798No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCCT50.1665556295802798No Hit
GCAAAAGTTCTCAGGGATGTCCCCCCATGTACTCTGCGTTGATACCACTG40.13324450366422386Clontech SMART CDS Primer II A (95% over 23bp)
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.13324450366422386No Hit
GTGCTGTCTCTCTTTGATGGAATCGTGCCTCCTTCTCTTTTGGTTGTTAC40.13324450366422386No Hit
GCAAAAGTTCTCAGGGATGTCTGCGTTGATACCACTGCTTCCCATGTACT40.13324450366422386Clontech Universal Primer Mix Long (95% over 24bp)
GGTGTTTGGGATGGAATGGTGATCCACACAGGTGGTTCGCAGAAGCAGCA40.13324450366422386No Hit
GCAAAAGTTCTCAGGGATGTCCGCGTTGATACCACTGCTTCCCATGTACT40.13324450366422386Clontech Universal Primer Mix Long (95% over 21bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.13324450366422386No Hit
GCAAAAGTTCTCAGGGATGTCCGCTGCGTTGATACCACTGCTTCCCATGT40.13324450366422386Clontech Universal Primer Mix Long (95% over 23bp)
CTCCAAGTAACGGTGCTGAGAAAGCAGAAATGAGTTCAGCAAAGTGAGGA40.13324450366422386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)