FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945
Total Bases571.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA245562.23067173637516No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA2937.427122940430925No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC1062.6869455006337137No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC892.256020278833967No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC561.4195183776932827No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG411.0392902408111533No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG350.8871989860583016No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA290.7351077313054499No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.6590621039290241No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC180.45627376425855515Clontech SMART CDS Primer II A (95% over 24bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG170.4309252217997465No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.4055766793409379No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT150.38022813688212925No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.32953105196451205No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT120.3041825095057034Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.3041825095057034No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG120.3041825095057034No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.2788339670468948No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT100.25348542458808615No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA100.25348542458808615No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA90.22813688212927757No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG90.22813688212927757No Hit
GTGCTGTCTCTCTTTGATGGAATCCTCTGCGGTGGCACTATTGTTGTCAT80.20278833967046894No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA80.20278833967046894No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG80.20278833967046894No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG80.20278833967046894No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT70.17743979721166034No Hit
GCAAAAGTTCTCAGGGATGTCCACATTGAGGCATTGCATAAATGTGTAAT60.1520912547528517No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT60.1520912547528517Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA60.1520912547528517No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA60.1520912547528517No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTCTCTTTTGGTTGTTACGCA60.1520912547528517No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.1520912547528517No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC60.1520912547528517No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCGGGGG50.12674271229404308Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGGAATGGTGATCCACGTAGGTGGTTCGCAGAAGCAGCA50.12674271229404308No Hit
GCAAAAGTTCTCAGGGATGTCGGAGATGTACTGGGGGCTGTATAGCCCAC50.12674271229404308No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC50.12674271229404308No Hit
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGTTACGC50.12674271229404308No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAACAGCA40.10139416983523447No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT40.10139416983523447Clontech SMART CDS Primer II A (96% over 25bp)
GCAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGCA40.10139416983523447No Hit
GGTGTTTGGGATGGAATGGTGATCTACGCAGGTGGTTCGCAGAAGCAGCA40.10139416983523447No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC40.10139416983523447No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA40.10139416983523447No Hit
GCAAAAGTTCTCAGGGATGTCGGAAGAATAGGGCGTGGCAGCCACCAGAG40.10139416983523447No Hit
GCAAAAGTTCTCAGGGATGTCCAAGATCAGATGGTTGTAGATGTGTGGTA40.10139416983523447No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA40.10139416983523447No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA40.10139416983523447No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGTAGAAGCAGCA40.10139416983523447No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC40.10139416983523447No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGCTCGCAGAAGCAGCA40.10139416983523447No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTGGTTGTTACGCA40.10139416983523447No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA40.10139416983523447No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)