FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9976
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT577657.898957497995184No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA186418.68484362469928No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1631.6339214113873297No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1401.4033680834001603No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1211.2129109863672816Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA470.471130713712911No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA380.3809141940657578No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA350.35084202085004007Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA300.30072173215717724No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT280.2806736166800321No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.2706495589414595No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC260.2606255012028869Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.24057738572574178No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA240.24057738572574178No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC240.24057738572574178No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT230.2305533279871692No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.2305533279871692No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.22052927024859664No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.22052927024859664No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.22052927024859664No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT200.20048115477145148No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT200.20048115477145148No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT200.20048115477145148No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA190.1904570970328789No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA170.17040898155573375No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT160.16038492381716118No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT160.16038492381716118No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.15036086607858862No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT150.15036086607858862No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT130.13031275060144346No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT130.13031275060144346No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.11026463512429832No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.11026463512429832No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.11026463512429832No Hit
TCCTATCAGTGGCCGCAAAGAAGACGAAAAAGTCGCTGGAGTCGATCAAC110.11026463512429832No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT110.11026463512429832No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC110.11026463512429832No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA110.11026463512429832No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT110.11026463512429832No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC100.10024057738572574Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT100.10024057738572574No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT100.10024057738572574No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA100.10024057738572574No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTAT100.10024057738572574No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)