FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4701
Total Bases681.3 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG234149.79791533716231No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA2014.2756860242501595No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1813.850244628802382No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1803.8289725590299937No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC1483.14826632631355No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA901.9144862795149968No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT521.1061476281642204No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT390.8296107211231653No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT390.8296107211231653No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT340.723250372261221Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA340.723250372261221No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT330.7019783024888321No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT280.5956179536268879Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT260.5530738140821102No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT210.4467134652201659No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC210.4467134652201659Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG180.3828972559029994No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG180.3828972559029994No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT170.3616251861306105No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAC160.34035311635822163No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG150.3190810465858328No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT150.3190810465858328No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT140.2978089768134439No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA130.2765369070410551No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT130.2765369070410551No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA130.2765369070410551No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT130.2765369070410551No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA110.2339927674962774No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC110.2339927674962774No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCT110.2339927674962774No Hit
GCAAAAGTTCTCAGGGATGTGGTGCCTCCTTCTCTTTTGGTTGTTACGCA110.2339927674962774No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA100.21272069772388852Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTTACGCAG90.1914486279514997No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG90.1914486279514997No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA90.1914486279514997No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT80.17017655817911082Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC80.17017655817911082No Hit
GCAAAAGTTCTCAGGGATGTGATGATTGATGCTTACGCAGACCAAGTCTC80.17017655817911082No Hit
GCAAAAGTTCTCAGGGATGTCCAGGCTTTTTCATGGTTTAGTTCCTCACA80.17017655817911082No Hit
GCAAAATTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT70.14890448840672196No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCCG70.14890448840672196No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTACGCCTG60.12763241863433314No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC60.12763241863433314No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA60.12763241863433314Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCAAAGATCAGATGGTTGTAGATACGTGGCA60.12763241863433314No Hit
GCAAAAGTTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG60.12763241863433314No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTTACGC50.10636034886194426No Hit
GCAAAAGTTCTCAGGGATGTGGTCAGCGCATCCTGCAGGGCTTCTCCTCC50.10636034886194426No Hit
GCAAAAGTTCTCAGGGATGTCCCATAGTACTTGCTGGTCTCAAATTTCCA50.10636034886194426No Hit
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACATAGAGGG50.10636034886194426No Hit
GCAAAAGTTCTCAGGGATGTCAAAGATCAGATAGTTGTAGACATGCGGCA50.10636034886194426No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACCTGCGCCTG50.10636034886194426No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA50.10636034886194426No Hit
GCAAAAGTTCTCAGGGATGTGTTACATGGGCTTTCTTGAAACGCAAGCCG50.10636034886194426No Hit
GCAAAAGTTCTCAGGGTGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT50.10636034886194426No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC50.10636034886194426No Hit
GTGCTGTCTCTCTTTGATGTTACGCAGACCAAGTCTCTGCTACCGTACGT50.10636034886194426No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT50.10636034886194426No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG50.10636034886194426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)