FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6237
Total Bases904.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA221335.481802148468816No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA132921.30832130832131No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT68811.030944364277698No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG1973.1585698252364915No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1422.2767356100689433No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1362.1805355138688474No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA951.5231681898348564No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC671.074234407567741No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG610.9780343113676447No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA480.7696007696007696No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG340.5451338784672118No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA330.5291005291005291No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA290.46496713163379827No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACTGCTTC280.44893378226711567Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT250.4008337341670675No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC250.4008337341670675No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.3848003848003848No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC240.3848003848003848No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG220.3527336860670194No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA200.320666987333654No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT190.3046336379669713No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA110.1763668430335097No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC100.160333493666827No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACCCCATG100.160333493666827No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT100.160333493666827No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.160333493666827No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC100.160333493666827No Hit
GTGCTGTCTTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC90.1443001443001443No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGATGGTTCGCAGAAGCAGCA90.1443001443001443No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC80.1282667949334616No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACCCTGTGATGATTGATGCTTACG80.1282667949334616No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG80.1282667949334616No Hit
GCAAAAGTTCTCAGGGATGTCCGGAAAGGGAACCCAGGTTAGTGTGGGGA70.11223344556677892No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC70.11223344556677892No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT70.11223344556677892Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.11223344556677892No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGATTACGCAGACCAAG70.11223344556677892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)