FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10691
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT328430.717425872228976No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA293027.406229538864462No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA6546.117294920961556No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA6466.0424656252923015No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC2412.2542325320362924No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2162.0203909830698716Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA1571.46852492750912No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC1161.0850247872041905No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT1141.0663174632868768No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT910.8511832382377701Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC590.551866055560752Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG560.5238050696847816No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG420.3928538022635862No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT310.28996352071836123Clontech SMART CDS Primer II A (96% over 25bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.25254887288373395No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.24319521092507718No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT260.24319521092507718No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.23384154896642037No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC240.22448788700776354No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA220.2057805630904499No Hit
GTGCTGTCTCTCTTTGAGGAATCGCTACAGATCCAGGAGTGGGGCCCATT210.1964269011317931No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT200.18707323917313629No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT180.16836591525582265No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA170.15901225329716584No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTGGCTCTCGAGCAGGCATGCCTCA170.15901225329716584No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC160.14965859133850903No Hit
GTGCTGTCTTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT140.1309512674211954No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA140.1309512674211954Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAACAGCTGGCTCTCGAGCAGGCATGCCTCA140.1309512674211954No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGATCCAGGAGTGGGGCCCATT140.1309512674211954No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT130.12159760546253859No Hit
GTGCTGTCTCTCTTGGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT130.12159760546253859No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA130.12159760546253859No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG130.12159760546253859No Hit
GTGCTGCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT130.12159760546253859No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.12159760546253859No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATCTGGGACTCTGCGTTGATACCAC130.12159760546253859No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA120.11224394350388177Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA120.11224394350388177No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.11224394350388177No Hit
GCAAAAGTTCTCAGGGATGTCCCATGTACTCTGCGTTGATACCACTGCTT110.10289028154522495Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAG110.10289028154522495No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA110.10289028154522495No Hit
GTGCTTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT110.10289028154522495No Hit
GTGCTGTCCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT110.10289028154522495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)