FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9299
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC467950.31723841273256No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA3573.8391224862888484No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT3153.387461017313689Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1952.096999677384665No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA1882.0217227658888053No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1611.7313689644047745No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC1361.4625228519195612No Hit
GCAAAAGTTCTCAGGGATGTAACTCTGCGTTGATACCACTGCTTCCCATG1291.3872459404237016Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT991.0646306054414454Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG870.9355844714485427No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT850.9140767824497258No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA840.9033229379503173No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA790.8495537154532745No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA770.8280460264544576No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG760.8172921819550489No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC700.7527691149585977Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA640.6882460479621465No Hit
GCAAAAGTTCTCAGGGATGCAACTCTGCGTTGATACCACTGCTTCCCATG620.6667383589633294Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA590.6344768254651038No Hit
GCAAAAGTTCTCAGGGATGTCAACTCTGCGTTGATACCACTGCTTCCCAT570.6129691364662867Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA530.5699537584686525No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT350.37638455747929883Clontech SMART CDS Primer II A (96% over 25bp)
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA340.3656307129798903No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA320.34412302398107325No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT320.34412302398107325No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA310.3333691794816647No Hit
GCAAAAGTTCTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAG300.32261533498225614No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA280.3011076459834391No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA270.29035380148403056No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG250.26884611248521345No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA210.22583073448757932Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGT180.19356920098935368No Hit
GCAAAAGTTCTCAGGGATGTCTGCGTTGATACCACTGCTTCCCATGTACT170.18281535648994515Clontech Universal Primer Mix Long (95% over 24bp)
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG170.18281535648994515No Hit
GCAAAAGTTCTCAGGGATGTCACTCTGCGTTGATACCACTGCTTCCCATG170.18281535648994515Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGGCAACTCTGCGTTGATACCACTGCTTCCCAT170.18281535648994515Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA160.17206151199053663No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG150.16130766749112807No Hit
GCAAAAGTTCTCAGGGATGTCCCATGTACTCTGCGTTGATACCACTGCTT150.16130766749112807Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT150.16130766749112807No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA140.15055382299171954No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA130.13979997849231102No Hit
GCAAAATTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA120.12904613399290246No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA110.11829228949349392No Hit
GTAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC110.11829228949349392No Hit
GCAAAAGTTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC110.11829228949349392No Hit
GCAAAAGTTCTCAGGGATGTAACTCTGCGTTGATACCACTGCTTCCATGT110.11829228949349392Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTCTCTTTTGGTTGTTACGCAGAC110.11829228949349392No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC110.11829228949349392No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACCCCATGTACTCT100.10753844499408538No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA100.10753844499408538Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTCTCTCTTTGATGGACAGCCCCCCCATGTACTCTGCGTTGATAC100.10753844499408538No Hit
GCAAAAGTTCTCAGGGATGTAACTCTGCGTTGATACCACCCCATGTACTC100.10753844499408538No Hit
GTGCTGTCTCTCTTTGATGGACCCCCATGTACTCTGCGTTGATACCACTG100.10753844499408538Clontech SMART CDS Primer II A (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)