FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9155
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length138-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT342637.42217367558711No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA117612.845439650464227No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA103611.316220644456582No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT6336.914254505734571No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3023.298743855816494Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1251.3653741125068268Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1071.1687602403058437No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC951.0376843255051884No Hit
GCATCAATCATCACAGGGTTGCTGAGGACATCCCTGAGAACTTTTGCCCT780.85199344620426No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC640.6990715456034954Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA500.5461496450027308Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA460.5024576734025124No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC380.4150737302020754Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC370.4041507373020207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC360.3932277444019661No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC330.3604587657018023No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA270.2949208083014746No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.28399781540142No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA230.2512288367012562No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.2403058438012015No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG220.2403058438012015No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA190.2075368651010377No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT180.19661387220098306No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA180.19661387220098306No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA180.19661387220098306No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.19661387220098306No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCTATG170.18569087930092845No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG170.18569087930092845No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC170.18569087930092845No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT150.1638448935008192No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA140.1529219006007646No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT130.14199890770071No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT130.14199890770071No Hit
CAACCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAG130.14199890770071No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA120.13107591480065536No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT120.13107591480065536No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT120.13107591480065536No Hit
CATCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAGA120.13107591480065536No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG120.13107591480065536No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.12015292190060076No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.12015292190060076No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA110.12015292190060076No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG110.12015292190060076No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA100.10922992900054614No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT100.10922992900054614No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA100.10922992900054614No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA100.10922992900054614No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA100.10922992900054614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)