FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273
Total Bases764.3 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA182434.59131424236677No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA89116.897401858524557No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT4728.951261141665086No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA1733.2808647828560593No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1001.8964536317087048Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT851.611985586952399No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC801.5171629053669637No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC741.4033756874644416No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC601.1378721790252229Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACTAGGCGGAAAGAAGACGTCGTCGTGGCG571.0809785700739616No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA531.0051204248056136Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC400.7585814526834819Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA370.7016878437322208No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA350.6637587710980467No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA320.6068651621467855No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA290.5499715531955244Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT280.5310070168784373No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.49307794424426327No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA250.4741134079271762No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA250.4741134079271762No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA200.37929072634174094No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC200.37929072634174094Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC200.37929072634174094No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC200.37929072634174094No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG180.3413616537075669No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.3413616537075669No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA180.3413616537075669No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA170.3223971173904798No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT160.30343258107339277No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA150.2844680447563057No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG140.26550350843921866No Hit
CAACCAAAAGAGAAGGAGGCACCACATCCCTGAGAACTTTTGCCCTCTAT110.20860989948795752No Hit
GCATCAATCATCACAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGT110.20860989948795752No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA100.18964536317087047No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA90.17068082685378344No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA80.15171629053669639No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA70.13275175421960933No Hit
CAACCAAAAGAGAAGGAGGCACCAACATCCCTGAGAACTTTTGCCCTCTA70.13275175421960933No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGGCAGCACCATCAAAGA70.13275175421960933No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA70.13275175421960933No Hit
CAACCAAAAGAGAAGGAAAATAGCAGATTTCTGAGCAGCGGTTTGTGAAG60.11378721790252229No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA60.11378721790252229No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAGTGAAAGGAGGCA60.11378721790252229No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA60.11378721790252229No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGGGGCA60.11378721790252229No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG60.11378721790252229No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCTCTATGTAGAAC60.11378721790252229No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC60.11378721790252229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)