FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7929
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA452457.05637533106318No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2533.1908185143145418Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA2202.7746247950561234No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1581.9926850800857612No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA1431.80350611678648No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1351.70261066969353No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1241.563879429940724No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1171.4755959137343928Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT1171.4755959137343928No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1151.4503720519611554Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1111.3999243284146803No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC871.0972379871358307Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA660.8323874385168369No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA560.7062681296506496No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC550.6936561987640308No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA500.630596544330937No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA490.6179846134443183Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA440.5549249590112246No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA440.5549249590112246No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA400.5044772354647497No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT310.390969857485181No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC300.37835792659856227Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC300.37835792659856227No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA270.340522133938706No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT260.32791020305208723No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA250.3152982721654685No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.2774624795056123No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.2774624795056123No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.22701475595913734No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGGGGCA170.2144028250725186No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA160.20179089418589985No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA140.1765670324126624No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT140.1765670324126624No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA140.1765670324126624No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA120.1513431706394249No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA100.1261193088661874No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA90.11350737797956867No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGGAAGGAGGCA90.11350737797956867No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGTCA90.11350737797956867No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGGACAAAATGAAAGGAGGCA90.11350737797956867No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCG90.11350737797956867No Hit
GGGCAAAGGGTGAAGGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA90.11350737797956867No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAGAGGAGGCA90.11350737797956867No Hit
GGGCAAAGGGTGAAGAAAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA80.10089544709294992No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA80.10089544709294992No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGTAAATGAACAAAATGAAAGGAGGCA80.10089544709294992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)