FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20755
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA885342.65478198024573No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT586928.27752348831607No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA18268.797880028908697No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG5392.596964586846543No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG2151.0358949650686582No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG2050.987713803902674No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT1200.5781739339918092No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG890.42881233437725846No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG880.4239942182606601No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG880.4239942182606601No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG760.3661768248614792No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACTGCTTC560.26981450252951095Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC450.21681522524692848No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG450.21681522524692848No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG420.2023608768971332No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA410.19754276078053482No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT400.1927246446639364Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG330.15899783184774752No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA320.15417971573114914No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA320.15417971573114914No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA310.1493615996145507No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA310.1493615996145507No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA280.13490725126475547No Hit
GTGCTGTCTCTCTTTGAGGAATCGCTACAGATCCAGGAGTGGGGCCCATT270.13008913514815706No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA270.13008913514815706No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC270.13008913514815706No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT270.13008913514815706No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT260.12527101903155866No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGATCCAGGAGTGGGGCCCATT260.12527101903155866No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA260.12527101903155866No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT240.11563478679836184No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA230.11081667068176342No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGATCCAGGAGTGGGGCCCATT220.10599855456516502No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT210.1011804384485666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)