FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11302
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA428237.887099628384355No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA236220.8989559370023No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC9698.573703769244382No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA3242.8667492479207217No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1621.4333746239603609Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1601.4156786409485047No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1491.318350734383295No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1211.0706069722173066No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1211.0706069722173066No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA1141.0086710316758096No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC700.6193594054149708No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT630.5574234648734737No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG520.46009555830826404No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT490.4335515837904796Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG490.4335515837904796No Hit
GCAAAAGTTCTCAGGGATGTGAGAGCAACTCTGCGTTGATACCACTGCTT460.4070076092726951Clontech SMART CDS Primer II A (95% over 24bp)
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA460.4070076092726951No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG440.3893116262608388No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.3273756857193417No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA370.3273756857193417No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA370.3273756857193417No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.26543974517784463No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC300.26543974517784463Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA290.2565917536719165No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.23004777915413202No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA250.22119978764820386No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT230.20350380463634754No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA230.20350380463634754No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA190.16811183861263493No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.16811183861263493No Hit
GCAAAAGTTCTCAGGGATGTGAGAGCAACTCTGCGTTGATACCACCCCAT180.15926384710670677No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA180.15926384710670677No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA160.14156786409485048No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.13271987258892232No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA150.13271987258892232No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT140.12387188108299416Clontech SMART CDS Primer II A (96% over 25bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.12387188108299416No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.11502388957706601No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA130.11502388957706601No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA130.11502388957706601No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA130.11502388957706601No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.10617589807113785No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT120.10617589807113785No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA120.10617589807113785No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCACTGCTTCCCA120.10617589807113785Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA120.10617589807113785No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)