FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12997
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA537341.34030930214665No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA227317.48865122720628No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC7826.016773101484958No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA6134.716473032238209No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2802.1543433099946143No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1391.0694775717473264No Hit
GCAAAAGTTCTCAGGGATGTCGAAGACGAGCAACTCTGCGTTGATACCAC1270.9771485727475571No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1230.9463722397476341No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1230.9463722397476341No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC900.6924674924982688No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT840.6463029929983842Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG600.46164499499884587No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT590.45395091174886515Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG540.4154804954989613No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA450.3462337462491344No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG440.33853966299915367No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.3077633299992306No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.23082249749942294No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA290.22312841424944216No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG290.22312841424944216No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC270.20774024774948066Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.18465799799953836No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG230.17696391474955758No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.16157574824959606No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC210.16157574824959606No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA200.1538816649996153No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA190.14618758174963453No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.14618758174963453No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA190.14618758174963453No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG190.14618758174963453No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTTCCGAAAACCAACAAAATAGAAC190.14618758174963453No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTTACGCA180.13849349849965376No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA180.13849349849965376No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT180.13849349849965376No Hit
GCAAAAGTTCTCAGGGATGTCCTTCCAGGAACACAGCCTCCTTCTCTTTT180.13849349849965376No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA180.13849349849965376No Hit
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAT170.13079941524967298No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC160.12310533199969223No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.11541124874971147No Hit
GCAAAAGTTCTCAGGGATGTCCAGGCTTTTTCATGGTTTAGTTCCTCACA150.11541124874971147No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA140.1077171654997307No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCGC140.1077171654997307No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGTTACGCAGACC140.1077171654997307No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA140.1077171654997307No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.10002308224974993No Hit

[FAIL]Adapter Content

Adapter graph