FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18113
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length136-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1099160.68017446033236No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC239613.228068238281898No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA8604.747971070501849No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCACTGCTTCC1510.833655385634627Clontech Universal Primer Mix Long (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG1480.8170926958538067No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1470.8115717992601998No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG1170.6459449014519958No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1140.6293822116711754No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA1060.585215038922321No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG950.5244851763926462No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCACCCCATGT920.5079224866118257No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACTGCTTCC900.49688069342461216Clontech Universal Primer Mix Long (96% over 25bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG830.45823441726936454No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG540.2981284160547673No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG490.2705239330867333No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA470.2594821398995197No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT450.24844034671230608No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG440.24291945011869928No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACCCCATGT430.23739855352509245No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.23739855352509245No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA380.20979407055705845No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA370.20427317396345168No Hit
GCAAAAGTTCTCAGGGATGTCCGCAACTCTGCGTTGATACCACTGCTTCC360.19875227736984485Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA350.19323138077623805No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG330.18218958758902445No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA320.17666869099541765No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.16010600121459725No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCCCATGTACT290.16010600121459725No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.14906420802738365No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA260.14354331143377685No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA250.13802241484017005No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG240.13250151824656323No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG240.13250151824656323No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA230.12698062165295643No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT210.11593882846574284No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCGGAAGCAGCA210.11593882846574284No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA200.11041793187213603No Hit
GCAAAAGTTCTCAGGGATGTTAGCAACTCTGCGTTGATACCATCCCATGT200.11041793187213603No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA190.10489703527852923No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA190.10489703527852923No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA190.10489703527852923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)