FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4572
Total Bases661.9 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA90319.750656167979002Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA72615.879265091863518No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA46710.214348206474192No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT3156.889763779527559No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2735.971128608923885Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC821.7935258092738406Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC761.6622922134733158No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC641.399825021872266No Hit
CAACCAAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTA531.1592300962379702No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC521.1373578302712162Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC370.8092738407699038No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA350.7655293088363955No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA330.7217847769028871No Hit
GCATCAATCATCACAGGGTTCATGGGAAGCAGTGGTATCAACGCAGAGTA330.7217847769028871Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA290.6342957130358705Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTTACATGGGAAGCAGTGGTATCAACGCAGAGT250.5468066491688539Clontech Universal Primer Mix Long (100% over 23bp)
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT250.5468066491688539No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT250.5468066491688539No Hit
CAACCAAAAGAGAAGGAGGCACCCTCTATGTAGAACCATGTCGTCAGTGT240.5249343832020997No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC230.5030621172353456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA220.4811898512685914Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC200.4374453193350831Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGCTGC190.41557305336832895No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG180.39370078740157477No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC160.3499562554680665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG150.32808398950131235No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA150.32808398950131235No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT150.32808398950131235No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC140.30621172353455817No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA140.30621172353455817No Hit
CAACCAAAAGAGAAGGAGGCACCACATCCCTGAGAACTTTTGCCCTCTAT130.28433945756780404No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCTATG120.26246719160104987No Hit
GCATCAATCATCACAGGGTACATGGGGAAGCAGTGGTATCAACGCAGAGT120.26246719160104987Clontech Universal Primer Mix Long (100% over 23bp)
GCATCAATCATCACAGGGTTGGGGGGAAGCAGTGGTATCAACGCAGAGTA110.2405949256342957Clontech Universal Primer Mix Long (96% over 27bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT110.2405949256342957No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG110.2405949256342957No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA100.21872265966754156No Hit
CAACCAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT100.21872265966754156No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGTGGT100.21872265966754156Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACATCCCTGAGAACTTTTGCCCTCTATGTAGAACCATG90.19685039370078738No Hit
GCATCAATCATCACAGGGTTGAGGGGAAGCAGTGGTATCAACGCAGAGTA90.19685039370078738Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT90.19685039370078738No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGCT90.19685039370078738Clontech Universal Primer Mix Long (95% over 22bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA80.17497812773403326No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA80.17497812773403326Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGACATCCCTGAGAACTTTTGCCCTCTATG80.17497812773403326No Hit
CAACCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAG80.17497812773403326No Hit
GCATCAATCATCACAGGGTACATGGAAAGCAGTGGTATCAACGCAGAGTA80.17497812773403326Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.15310586176727908No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTT70.15310586176727908Clontech Universal Primer Mix Long (100% over 23bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGGA70.15310586176727908Clontech Universal Primer Mix Long (96% over 25bp)
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.15310586176727908No Hit
GCATCAATCATCACAGGGTTGGGGGGGAAGCAGTGGTATCAACGCAGAGT70.15310586176727908Clontech Universal Primer Mix Long (96% over 27bp)
CAACCAAAAGAGAAGGAGGCACCAACATCCCTGAGAACTTTTGCCCTCTA60.13123359580052493No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA60.13123359580052493No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGTA60.13123359580052493No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGGGTC60.13123359580052493Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGC60.13123359580052493Clontech Universal Primer Mix Long (95% over 23bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGGCAGCACCATCAAAGA60.13123359580052493No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCACCCTCC60.13123359580052493No Hit
CAACCAAAAAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.10936132983377078No Hit
CATCAAAGAGAGACAGCACCCTCTATGTAGAACCATGTCGTCAGTGTAGA50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTGCCCTCTAT50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGGGAC50.10936132983377078Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTAT50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACACTCTATGT50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGGACATCCCTGAGAACTTTTGCCCTCTATGTAG50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGGCACCCGGAAGCAGTGGTATCAACGCAGAGTA50.10936132983377078Clontech Universal Primer Mix Long (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA50.10936132983377078No Hit
CAACCAAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.10936132983377078No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.10936132983377078No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCACCCTCT50.10936132983377078No Hit
CAACCAAAAGAGAGACAGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.10936132983377078No Hit

[FAIL]Adapter Content

Adapter graph