FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177
Total Bases315.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT35916.49058337161231Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1828.360128617363344No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1074.915020670647681No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTTACGC863.9503904455672942No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC713.261368856224162Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTTACGCAGA663.031694993109784No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATTACGCAGACCAAGTCTCTG652.9857602204869087No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT542.480477721635278Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT452.067064768029398No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTG431.9751952227836471No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT421.929260450160772Clontech SMART CDS Primer II A (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA311.423977951309141Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCCATGTACTCTGCGTTGATACCACTGCTT271.240238860817639Clontech SMART CDS Primer II A (96% over 26bp)
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTTACGCAGACCA241.1024345429490123No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCAATGACAGCAACTCTGCGTTGAT231.056499770326137No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTTACGCAGACCA221.0105649977032614No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTTACGCAGACCAAG190.8727606798346348No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATTACGCAGACCAAGTCTCTG190.8727606798346348No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACTGCTTCC150.6890215893431328Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCGCAACTCTGCGTTGATACCACTGCTTCC140.6430868167202572Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGACCAATGACAGCAACTCTGCGTTGATACCA130.5971520440973818No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC120.5512172714745062No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTTACGCAGACCAAGTCTCT120.5512172714745062No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATCTGGGACTCTGCGTTGATACCAC120.5512172714745062No Hit
GTGCCTCCTTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTA110.5052824988516307No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATTACGCA110.5052824988516307No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT110.5052824988516307No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG110.5052824988516307No Hit
GTGCTGTCTCTCTTTGATGGACAGCCCCCCCATGTACTCTGCGTTGATAC100.45934772622875514No Hit
GCAAAAGTTCTCAGGGATGTCCAGCAACTCTGCGTTGATACCACTGCTTC90.41341295360587965Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGACCCCATGTACTCTGCGTTGATACCACTGC90.41341295360587965Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTCCCATGTA90.41341295360587965Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCCATG80.3674781809830041Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA80.3674781809830041No Hit
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCCCATGTACT80.3674781809830041No Hit
GTGCTGTCTCTCTTTGATGGACCCCCATGTACTCTGCGTTGATACCACTG80.3674781809830041Clontech SMART CDS Primer II A (95% over 23bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA80.3674781809830041Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTCAGGGATGACAGCAACTCTGCGTTGATACCACTGCTTCC70.3215434083601286Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTTGGTTGTTACGCAGACCAAGTCTCTGCTACCGTAA70.3215434083601286No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTCAGGCTTTTTCATGGTTTAG60.2756086357372531No Hit
CTCCAAGTAACGGTGCTTTCTCTTCACCCTTTGCCCTTACGCAGACCAAG60.2756086357372531No Hit
GCAAAAGTTCTCAGGGATGTCACTGCTTCCCATGTACTCTGCGTTGATAC60.2756086357372531No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA50.22967386311437757No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTCCCCATGT50.22967386311437757Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAAAAGTTCTCAGGGATGTCAGCAACTCTGCGTTGATACCACCCCATGT50.22967386311437757No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTA50.22967386311437757No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATCCCCCAT40.18373909049150206No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTCTCTTCACCCTTTGCCCTTACGC40.18373909049150206No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT40.18373909049150206Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GCAAAAGTTCTCAGGGATGGCAGCAACTCTGCGTTGATACCACTGCTTCC40.18373909049150206Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACCCCATGTACTCT40.18373909049150206No Hit
GCAAAAGTTCTCAGGGATGTCCGCAACTCTGCGTTGATACCACCCCATGT40.18373909049150206No Hit
GTGCTGTCTCTCTTTGATGGAAGTTTAGCACCCCATGTACTCTGCGTTGA40.18373909049150206No Hit
GCAAAAGTTCTCAGGGATGTCCCTGCTTCCCATGTACTCTGCGTTGATAC40.18373909049150206No Hit
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCACTTTTTCATGGTTAGTT40.18373909049150206No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCAATGT40.18373909049150206Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCATG30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCTGGT30.13780431786862654Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAGTAACGGTGCGGCCACTGATAGGATTACGCAGACCAAGTCTCTGC30.13780431786862654No Hit
CTCCAAGTAACGGTGCTGTCTCTCACCCTTTGCCCTTACGCAGACCAAGT30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCAGGT30.13780431786862654Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGTACTCTGCGTTGATACCACTGCTTCCCATGT30.13780431786862654Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCATGT30.13780431786862654No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGTCAGGACCACCCATGTACTCTGCGTTGATAC30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTTCCATGT30.13780431786862654Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTCTCTTTTGGTTGTTACGCA30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGTCCGGCTTTTTCATGGTTTAGTTCCTCACAC30.13780431786862654No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC30.13780431786862654No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTCTC30.13780431786862654No Hit
GTGCTGTCTCTCTTTGATGGACGGCACCCCATGTACTCTGCGTTGATACC30.13780431786862654No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT30.13780431786862654No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGCCAGCAACTCTGCGTTGATACCACTGCTTCC30.13780431786862654Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGCCTCTC30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGTGAAAGCAGAAATGAGTTCAGCAAAGTGAGG30.13780431786862654No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC30.13780431786862654No Hit
GCAAAAGTTCTCAGGGATGTCCGTGCCTCCTTCTCTTTTGGTTGTTACGC30.13780431786862654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)