FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008147331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008147331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13763
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA796757.88708857080578No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC4203.0516602484923343No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4173.029862675288818Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC3872.811886943253651No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA3242.3541379059798007No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1771.286056819007484No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCTATGT1681.2206640993969338No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1501.089878660175834No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA1250.9082322168131948No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGTC1140.8283077817336337No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1080.7847126353266003No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1080.7847126353266003Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA930.6757247693090169No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCTCTATGTAGA880.6393954806364891No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA880.6393954806364891No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGA700.508610041415389No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCTCTATGTAGAAC680.494078325946378No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCTCTAT580.42141974860132236No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCTCTATGTAGAACCATGT540.39235631766330015No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCTCTA530.3850904599287946No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA490.3560270289907724No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA450.3269635980527501No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA440.31969774031824455No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA370.26883673617670567No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA320.23250744750417787No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT300.21797573203516676No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.18891230109714452No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA260.18891230109714452No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGGAAGGAGGCA250.18164644336263897No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA230.16711472789362783No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA230.16711472789362783No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC230.16711472789362783Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA220.15984887015912227No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA210.15258301242461672No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA210.15258301242461672No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCTCTAT210.15258301242461672No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA200.14531715469011117Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA190.1380512969556056No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGGGGCA190.1380512969556056No Hit
GGGCAAAGGGTGAAGGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA190.1380512969556056No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC190.1380512969556056Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGTCA180.13078543922110003No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCTCTATGTAGAAC180.13078543922110003No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCG170.1235195814865945No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA170.1235195814865945No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAGTGAAAGGAGGCA170.1235195814865945No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGGACAAAATGAAAGGAGGCA160.11625372375208894No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGTAAATGAACAAAATGAAAGGAGGCA160.11625372375208894No Hit
GGGCAAAGGGTGAAGAAAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA150.10898786601758338No Hit
GGGCAAAGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAAG150.10898786601758338No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCTCTATG140.10172200828307781No Hit
GGGCAAAGGGTGAAGAGAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.10172200828307781No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT140.10172200828307781No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAGAGGAGGCA140.10172200828307781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)